CAJ | 학술논문

背景:白色念珠菌生物膜是根管充填治疗预后的影响因素,茶多酚体外可能对白色念珠菌生物膜有抑菌作用,其抑菌机制可能通过影响或干扰相关耐药基因的表达而实现.目的:探讨根管充填材料加入茶多酚对白色念珠菌生物膜的抑制作用.方法:采用常量肉汤稀释法确定茶多酚体外对白色念珠菌生物膜的最小抑菌浓度,以此判断抑菌效果,然后用逆转录聚合酶链反应方法检测白色念珠菌耐药基因CDR1、CDR2和MDR1受茶多酚作用后的表达水平的变化.结果与结论:茶多酚对白色念珠菌生物膜的最小抑菌浓度为11.5 g/L,反转录聚合酶链反应结果表明,相关耐药基因CDR1、CDR2的表达水平随着药物浓度的梯度增加而降低,在浓度为23 g/L及更高时mRNA的表达水平受到完全抑制,而药物浓度变化对耐药基因MDR1的表达影响较小.茶多酚体外对白色念珠菌生物膜起到了抑制作用,并对实验所选相关耐药基因CDR1、CDR2的表达过程明显产生了抑制作用.
배경:백색념주균생물막시근관충전치료예후적영향인소,다다분체외가능대백색념주균생물막유억균작용,기억균궤제가능통과영향혹간우상관내약기인적표체이실현.목적:탐토근관충전재료가입다다분대백색념주균생물막적억제작용.방법:채용상량육탕희석법학정다다분체외대백색념주균생물막적최소억균농도,이차판단억균효과,연후용역전록취합매련반응방법검측백색념주균내약기인CDR1、CDR2화MDR1수다다분작용후적표체수평적변화.결과여결론:다다분대백색념주균생물막적최소억균농도위11.5 g/L,반전록취합매련반응결과표명,상관내약기인CDR1、CDR2적표체수평수착약물농도적제도증가이강저,재농도위23 g/L급경고시mRNA적표체수평수도완전억제,이약물농도변화대내약기인MDR1적표체영향교소.다다분체외대백색념주균생물막기도료억제작용,병대실험소선상관내약기인CDR1、CDR2적표체과정명현산생료억제작용.
@@@@BACKGROUND: Candida albicans biofilms can influence the prognosis of root canal fil ing, and tea polyphenols can in vitro inhibit the Candida albicans biofilm, while the antibacterial mechanism may play the role through influencing or interfering the expression of resistance gene. OBJECTIVE: To investigate the inhibitory effect of root canal fil ing material added with tea polyphenols on Candida albicans biofilm. METHODS: Constant broth dilution method was used to determine the minimal inhibitory concentration of tea polyphenols in vitro inhibited Candida albicans biofilms, in order to identify the inhibitory effect. Then the expressions of Candida albicans resistance genes CDR1, CDR2 and MDR1 were detected with reverse transcriptase polymerase chain reaction after inhibited with tea polyphenols. RESULTS AND CONCLUSION: The minimum inhibitory concentration of tea polyphenols used to inhibit Candida albicans biofilm was 11.5 mg/mL. Reverse transcriptase polymerase chain reaction results showed that expressions of related resistance genes CDR1 and CDR2 were decreased with the gradient increasing of drug concentration, and when the concentration reached to 25 mg/mL or above, the mRNA expression level was completely inhibited, while the concentration of the resistance drug has less effect on the expression of resistance drug MDR1. Tea polyphenols has inhibitory effect on Candida albicans biofilm, and has significant inhibitory effect on the expressions of experimental selected resistance genes CDR1 and CDR2.