CAJ | 학술논문

目的探讨Bcl-2、Bax在孤独症发病中的作用.方法孕12.5 d Sprague-Dawley孕鼠腹腔注射丙戊酸钠600 mg/kg建立子代孤独症模型大鼠,对照组注射同等剂量生理盐水.利用尼氏染色、免疫组化和图像分析技术观察比较出生后1 d、7 d、14 d、28 d和56 d两组大鼠脑部Bcl-2、Bax表达.结果尼氏染色:出生后1 d、7 d模型组神经元数量较少,出生14 d后剧增,出生后28 d、56 d仍高于对照组.免疫组化:出生后1~14 d两组Bcl-2、Bax表达均显著升高(P<0.001),出生28后表达趋于稳定(P>0.05);与对照组相比,模型组各日龄大鼠Bcl-2表达水平均显著降低(P<0.001);Bax于出生后1 d、7 d表达显著升高(P<0.001),出生后14 d表达显著降低(P<0.001),出生28 d后水平相似(P>0.05).对照组Bcl-2/Bax值出生后1 d最大,随年龄逐渐减小,出生14后趋近于1.模型组Bcl-2/Bax值出生后7 d最低,14 d最高,28 d后趋于稳定.结论孤独症模型大鼠大脑皮层神经元细胞抗凋亡能力下降,尤其在出生后早期.
목적탐토Bcl-2、Bax재고독증발병중적작용.방법잉12.5 d Sprague-Dawley잉서복강주사병무산납600 mg/kg건립자대고독증모형대서,대조조주사동등제량생리염수.이용니씨염색、면역조화화도상분석기술관찰비교출생후1 d、7 d、14 d、28 d화56 d량조대서뇌부Bcl-2、Bax표체.결과니씨염색:출생후1 d、7 d모형조신경원수량교소,출생14 d후극증,출생후28 d、56 d잉고우대조조.면역조화:출생후1~14 d량조Bcl-2、Bax표체균현저승고(P<0.001),출생28후표체추우은정(P>0.05);여대조조상비,모형조각일령대서Bcl-2표체수평균현저강저(P<0.001);Bax우출생후1 d、7 d표체현저승고(P<0.001),출생후14 d표체현저강저(P<0.001),출생28 d후수평상사(P>0.05).대조조Bcl-2/Bax치출생후1 d최대,수년령축점감소,출생14후추근우1.모형조Bcl-2/Bax치출생후7 d최저,14 d최고,28 d후추우은정.결론고독증모형대서대뇌피층신경원세포항조망능력하강,우기재출생후조기.
Objective To explore the role of Bcl-2 and Bax in pathogenesis of the autism. Methods Female Sprague-Dawley rats were given a single intraperitoneal injection of sodium valproate (VPA, 600 mg/kg) on 12.5 d after pregnancy, their offspring were as the model group;while the other pregnancy rats were given normal saline, their offspring were as the control group. Both groups were observed with the Nissl staining, immunohistochemistry of Bcl-2 and Bax and image analysis 1 d, 7 d, 14 d, 28 d, 56 d after birth. Results Compared with the control group, Nissl staining showed the number of cortical neurons decreased on 1 d and 7 d after birth in the model group, rapidly in-creased on 14 d after birth, and maintained in high level on 28d , 56 d after birth. For immunohistochemistry, the integrated optical density (IOD) of Bcl-2 and Bax decreased in cortex on 1~14 d after birth (P<0.001) in both groups, and were stable 28 d after birth (P>0.05). Com-pared with the control group, the IOD of Bcl-2 decreased much more at every time point (P<0.001) in the model group, but the IOD of Bax increased on 1 d, 7 d (P<0.001), decreased on 14 d (P<0.001), similar 28 after birth(P>0.05). The ratio of Bcl-2/Bax was the most on 1 d af-ter birth, and then decreased to approximately 1 in the control group, while it was the least on 7 d, most on 14 d, and decrease to less than 1 28 d after birth. Conclusion Apoptosis of cerebral cortex neurons increases in the autism model rats, especially in the early time.