CAJ | 학술논문

目的探讨早期干预对仔鼠脑组织NB-3的表达及其运动功能的影响.方法孕17~18 d Wistar大鼠45只腹腔注射脂多糖(LPS)380μg/kg连续2 d (LPS组),另15只注射同等剂量的无菌生理盐水(NS组).随机选取LPS组仔鼠80只,分为干预组40只(I组)和非干预组40只(NI组);随机选取NS组仔鼠40只为对照组.早期干预措施主要给予早期触摸与丰富康复训练.仔鼠出生后24 h内在LPS组和NS组随机取5只行脑组织HE染色.其余大鼠分别于出生后14 d、28 d行悬吊试验,1 d、7 d、14 d、21 d、28 d行脑组织免疫组织化学染色,观察NB-3的表达.结果脑组织NB-3的表达及悬吊试验评分三组间均有非常高度显著性差异(P<0.001).结论宫内感染可致脑损伤鼠NB-3表达增加,早期干预使脑损伤鼠NB-3表达持续增加,并改善其运动功能.
목적탐토조기간예대자서뇌조직NB-3적표체급기운동공능적영향.방법잉17~18 d Wistar대서45지복강주사지다당(LPS)380μg/kg련속2 d (LPS조),령15지주사동등제량적무균생리염수(NS조).수궤선취LPS조자서80지,분위간예조40지(I조)화비간예조40지(NI조);수궤선취NS조자서40지위대조조.조기간예조시주요급여조기촉모여봉부강복훈련.자서출생후24 h내재LPS조화NS조수궤취5지행뇌조직HE염색.기여대서분별우출생후14 d、28 d행현조시험,1 d、7 d、14 d、21 d、28 d행뇌조직면역조직화학염색,관찰NB-3적표체.결과뇌조직NB-3적표체급현조시험평분삼조간균유비상고도현저성차이(P<0.001).결론궁내감염가치뇌손상서NB-3표체증가,조기간예사뇌손상서NB-3표체지속증가,병개선기운동공능.
Objective To investigate the effect of early intervention on adhesion molecule NB-3 expressing in cerebral injured neonatal rat induced with intrauterine infection. Methods Pregnant rats were consecutively injected with lipopolysaccharide (LPS) 380 μg/kg or sa-line 17 d after gestation. The offspring from the LPS group accepted early intervention (I group, n=40) or not (NI group, n=40), and those for saline group were as control group (n=40). They were assessed with suspension test 14 d and 28 d after born, and the expression of were NB-3 were detected with immunohistochemistry 1 d, 7 d, 14 d, 21 d, 28 d after born. Results The expression of NB-3 was different among these 3 groups, as well as the scores of suspension test (P<0.001). Conclusion Intrauterine infection increases the expression of NB-3 of neo-natal rats with brain damage. The early intervention may increase persistently the expression of NB-3, and improve the motor function.