CAJ | 학술논문

目的比较电针不同经络穴组对局灶性脑梗死大鼠神经行为学及neurocan表达的干预效果.方法将健康成年雄性Sprague-Dawley大鼠85只随机分为5组:假手术组(n=5)、模型组(n=20)、水沟-百会组(n=20)、肝俞-肾俞组(n=20)和足三里-曲池组(n=20);模型组和各电针组线栓法制作大鼠大脑中动脉闭塞模型,随机再分为脑缺血1 d、3 d、7 d、14 d和21 d共5个亚组.神经行为学采用Longa评分,RT-PCR及免疫组化测定缺血侧皮质neurocan mRNA和蛋白表达.结果假手术组无神经功能障碍;各电针组大鼠在缺血3 d后神经行为学评分逐渐恢复,7~21 d时较模型组明显改善(P<0.05).模型组neurocan mRNA和蛋白表达在缺血1 d后逐渐增强,14 d达到峰值,21 d时有所下降,但仍维持高水平表达(P<0.01);各电针组在缺血3 d后neurocan mRNA和蛋白表达均较模型组低(P<0.05),其中水沟-百会和肝俞-肾俞组在缺血14 d时neurocan mRNA和蛋白表达较足三里-曲池组低(P<0.05).结论局灶性脑梗死大鼠缺血侧皮质neurocan mRNA和蛋白表达明显上调.急性期给予电针刺激能下调其表达水平,改善神经行为学评分;其中电针水沟-百会穴和肝俞-肾俞穴较足三里-曲池穴治疗效果更好.
목적비교전침불동경락혈조대국조성뇌경사대서신경행위학급neurocan표체적간예효과.방법장건강성년웅성Sprague-Dawley대서85지수궤분위5조:가수술조(n=5)、모형조(n=20)、수구-백회조(n=20)、간유-신유조(n=20)화족삼리-곡지조(n=20);모형조화각전침조선전법제작대서대뇌중동맥폐새모형,수궤재분위뇌결혈1 d、3 d、7 d、14 d화21 d공5개아조.신경행위학채용Longa평분,RT-PCR급면역조화측정결혈측피질neurocan mRNA화단백표체.결과가수술조무신경공능장애;각전침조대서재결혈3 d후신경행위학평분축점회복,7~21 d시교모형조명현개선(P<0.05).모형조neurocan mRNA화단백표체재결혈1 d후축점증강,14 d체도봉치,21 d시유소하강,단잉유지고수평표체(P<0.01);각전침조재결혈3 d후neurocan mRNA화단백표체균교모형조저(P<0.05),기중수구-백회화간유-신유조재결혈14 d시neurocan mRNA화단백표체교족삼리-곡지조저(P<0.05).결론국조성뇌경사대서결혈측피질neurocan mRNA화단백표체명현상조.급성기급여전침자격능하조기표체수평,개선신경행위학평분;기중전침수구-백회혈화간유-신유혈교족삼리-곡지혈치료효과경호.
Objective To compare the effects of electroacupuncture (EA) at different acupoints on the neuroethology and expression of neurocan after focal cerebral infarction in rats. Methods The healthy male adult Sprague-Dawley rats were randomly divided into sham group (n=5), model group (n=20), Shuigou-Baihui (SB) group (n=20), Ganshu-Shenshu (GS) group (n=20) and Zusanli-Quchi (ZQ) group. All the rats were modeled with middle cerebral artery occlusion (MCAO) except sham group, and were randomly divided into 5 sub-groups as 1 d, 3 d, 7 d, 14 d and 21 d after cerebral ischemia. Their neuroethology was evaluated with Longa's score, and the expressions of neuro-can mRNA and protein were detected with RT-PCR and immunohistochemistry in ischemic cortex. Results No dysfunction was found in the sham group. The neurological function recovered gradually 3 d after modeled, and improved significantly 7~21 d after modeled in each EA group compared with those in the model group (P<0.05). The expression of neurocan mRNA and protein gradually increased 1 d after mod-eled in the model group, peaked 14 d after modeled, and remained high level 21 d after modeled (P<0.01). The expressions of neurocan mRNA and protein were less in each EA group than in the model group 3 d after modeled (P<0.05), and were less in the SB group and GS group than in ZQ group (P<0.05). Conclusion The expression of neurocan increased significantly in ischemic cortex. EA may down-regu-late the expression of neurocan after acute cerebral infarction, and improve the neuroethology. EA at SB and GS is more effective than at ZQ.