河南科学
河南科學
하남과학
HENAN SCIENCE
2013年
4期
441-444
,共4页
周伏忠%冯菲%时翔明%孙玉飞%李锋
週伏忠%馮菲%時翔明%孫玉飛%李鋒
주복충%풍비%시상명%손옥비%리봉
γ-射线辐照%纳豆激酶酶活%活菌数%灭菌
γ-射線輻照%納豆激酶酶活%活菌數%滅菌
γ-사선복조%납두격매매활%활균수%멸균
γ-ray irradiation%nattokinase fibrinolytic activity%vital microbes%disinfection
对60钴γ-射线辐照前后的纳豆激酶(NK)制剂有效成分、酶活及活菌数等技术指标进行了实验研究,结果表明,不同剂量的60钴γ-射线辐照对纳豆激酶制剂中的总皂甙含量和纳豆激酶酶活影响不大,30 kGy时总皂甙含量仍可达到原始含量的105%~125%,NK酶活达到原始酶活的90%以上;纳豆芽孢杆菌、大肠菌群和金黄色葡萄球菌等活菌数对60钴γ-射线辐照十分敏感,5 kGy的60钴γ-射线辐照处理后纳豆芽孢杆菌、大肠菌群和金黄色葡萄球菌数量下降99%以上.
對60鈷γ-射線輻照前後的納豆激酶(NK)製劑有效成分、酶活及活菌數等技術指標進行瞭實驗研究,結果錶明,不同劑量的60鈷γ-射線輻照對納豆激酶製劑中的總皂甙含量和納豆激酶酶活影響不大,30 kGy時總皂甙含量仍可達到原始含量的105%~125%,NK酶活達到原始酶活的90%以上;納豆芽孢桿菌、大腸菌群和金黃色葡萄毬菌等活菌數對60鈷γ-射線輻照十分敏感,5 kGy的60鈷γ-射線輻照處理後納豆芽孢桿菌、大腸菌群和金黃色葡萄毬菌數量下降99%以上.
대60고γ-사선복조전후적납두격매(NK)제제유효성분、매활급활균수등기술지표진행료실험연구,결과표명,불동제량적60고γ-사선복조대납두격매제제중적총조대함량화납두격매매활영향불대,30 kGy시총조대함량잉가체도원시함량적105%~125%,NK매활체도원시매활적90%이상;납두아포간균、대장균군화금황색포도구균등활균수대60고γ-사선복조십분민감,5 kGy적60고γ-사선복조처리후납두아포간균、대장균군화금황색포도구균수량하강99%이상.
@@@@The nattokinase crudes were treated with γ-ray irradiation at different absorbed doses,and the total ginsenosides,nattokinase activity and the vital microbes were tested. It was found that the influences of theγ-ray irradiation on the total ginsenosides and nattokinase activity were slightly,while the lethal effect of γ-ray irradiation on the vital microbes such as Bacillus subtilus Natto,Staphylococcus aureus and coliform bacteria were seriously(decreased 99%). This studies provided some experimental data for irradiation methods applicated on the nattokinase crudes disinfection and preservation.
