CAJ | 학술논문

苘麻(Abutilon theophrasti Medic.)是锦葵科一年生草本植物,在医药业和农业方面均有较高的应用价值.实验通过组织培养技术,以苘麻小苗的茎段和叶片为外植体诱导产生愈伤组织,为苘麻的细胞培养和快繁奠定基础.实验结果表明:苘麻种子的最适消毒处理为0.1%升汞8 min;种子萌发培养基为蔗糖30 g/L+琼脂5.7 g/L;由茎段和叶片诱导愈伤组织阶段的最佳培养基为 MS 培养基+琼脂7 g/L+蔗糖30 g/L+NAA 0.8 mg/L+6-BA 3.0 mg/L.
경마(Abutilon theophrasti Medic.)시금규과일년생초본식물,재의약업화농업방면균유교고적응용개치.실험통과조직배양기술,이경마소묘적경단화협편위외식체유도산생유상조직,위경마적세포배양화쾌번전정기출.실험결과표명:경마충자적최괄소독처리위0.1%승홍8 min;충자맹발배양기위자당30 g/L+경지5.7 g/L;유경단화협편유도유상조직계단적최가배양기위 MS 배양기+경지7 g/L+자당30 g/L+NAA 0.8 mg/L+6-BA 3.0 mg/L.
@@@@A theophrasti Medic. is an annual Inner Mongolian herb with important value in medicine industry and agriculture. The purpose of this research is to induce callus tissue with the stem sections and leaves as explants, thereby lay a foundation for cell culture and rapid propagation. The results of this research contain: the most effective sterilization time for seeds is 8 min by 0.1% HgCl; the optimized medium for seed germination is sucrose 30 g/L + Agar 5.7 g/L; the best medium for callus induction with stem sections and leaves as explants is MS medium+Agar 7g/L+Sucrose 30 g/L+ NAA 0.8 mg/L+6-BA 3 mg/L.