CAJ | 학술논문

背景:目前放疗治疗脑胶质瘤效果不理想,可能因素有很多.目的:探讨脑胶质瘤中肿瘤干细胞的体外放射敏感性.方法:取脑胶质瘤细胞,接种于含生长因子的无血清培养基中培养,取细胞活力最强的位点扩增3-5代的肿瘤球细胞,给予不同X射线剂量照射,检测其细胞活力,以确定最适的放疗剂量.结果与结论:胶质瘤中不同部位的肿瘤细胞增殖活力有差异;8 Gy以上X射线剂量对脑肿瘤干细胞具有显著的杀伤作用.说明脑胶质瘤具有异质性,部位不同,脑肿瘤干细胞增殖活力不同;不同的放疗剂量对脑肿瘤干细胞有不同的影响.
배경:목전방료치료뇌효질류효과불이상,가능인소유흔다.목적:탐토뇌효질류중종류간세포적체외방사민감성.방법:취뇌효질류세포,접충우함생장인자적무혈청배양기중배양,취세포활력최강적위점확증3-5대적종류구세포,급여불동X사선제량조사,검측기세포활력,이학정최괄적방료제량.결과여결론:효질류중불동부위적종류세포증식활력유차이;8 Gy이상X사선제량대뇌종류간세포구유현저적살상작용.설명뇌효질류구유이질성,부위불동,뇌종류간세포증식활력불동;불동적방료제량대뇌종류간세포유불동적영향.
BACKGROUND:Radiotherapy effect on of brain glioma is not ideal, and there are various possible factors that influence the therapeutic effect. OBJECTIVE:To investigate the in vitro radiosensitivity of brain cancer stem cel s in brain glioma. METHODS:Glioma cel s were isolated and seeded into the serum-free medium containing growth factors for culture. Glioma cel s from the most active cel site were subcultured to 3-5 generations. Glioma cel vitality was assessed to determine the optimal dose of X-ray radiation. RESULTS AND CONCLUSION:Distinct difference in activities of proliferation could be found among cancer stem cel s from different sites of glioma. X-ray dosages above 8 Gy had notable kil ing effect on brain cancer stem cel s. Different irradiation dosage had different influence on cancer stem cel s.