CAJ | 학술논문

干细胞%干细胞培养与分化%糖皮质激素%神经胶质抗原2%神经祖细胞%增殖%凋亡%应激%地塞米松%海马%原代培养%神经发生%省级基金榦細胞%榦細胞培養與分化%糖皮質激素%神經膠質抗原2%神經祖細胞%增殖%凋亡%應激%地塞米鬆%海馬%原代培養%神經髮生%省級基金
간세포%간세포배양여분화%당피질격소%신경효질항원2%신경조세포%증식%조망%응격%지새미송%해마%원대배양%신경발생%성급기금
stem cells%stem cell culture and proliferation%glucocorticoid%neuron-glia antigen 2%neural progenitor cells%proliferation%apoptosis%stress%dexamethasone%hippocampus%primary culture%neurogenesis%provincial grants-supported paper

背景:体内研究显示,高浓度糖皮质激素可抑制大鼠内源性神经前体细胞增殖.而神经胶质抗原2蛋白聚糖阳性神经祖细胞(NG2细胞)是成熟中枢神经系统中最大的增殖细胞群体,具有多分化潜能.目的:观察糖皮质激素对体外培养的成熟大鼠海马由来的NG2细胞生存和增殖的影响.方法:原代及传代培养成年大鼠海马NG2细胞,以0,0.1,1,10,100μmol浓度糖皮质激素类药物地塞米松干预48 h后,采用乳酸脱氢酶分析法测定细胞活性,原位缺口末端标记技术(即TUNEL法)观察细胞凋亡情况,5’-溴脱氧尿嘧啶核苷掺入法鉴定细胞增殖状况.结果与结论:1,10,100μmol浓度地塞米松干预明显减少NG2细胞数,并显著增加TUNEL阳性细胞率,明显减少 BrdU 阳性细胞率.结果可见高浓度糖皮质激素能抑制 NG2细胞分裂增殖,并诱导细胞凋亡,从而明显减少NG2细胞数.
배경:체내연구현시,고농도당피질격소가억제대서내원성신경전체세포증식.이신경효질항원2단백취당양성신경조세포(NG2세포)시성숙중추신경계통중최대적증식세포군체,구유다분화잠능.목적:관찰당피질격소대체외배양적성숙대서해마유래적NG2세포생존화증식적영향.방법:원대급전대배양성년대서해마NG2세포,이0,0.1,1,10,100μmol농도당피질격소류약물지새미송간예48 h후,채용유산탈경매분석법측정세포활성,원위결구말단표기기술(즉TUNEL법)관찰세포조망정황,5’-추탈양뇨밀정핵감참입법감정세포증식상황.결과여결론:1,10,100μmol농도지새미송간예명현감소NG2세포수,병현저증가TUNEL양성세포솔,명현감소 BrdU 양성세포솔.결과가견고농도당피질격소능억제 NG2세포분렬증식,병유도세포조망,종이명현감소NG2세포수.
BACKGROUND:In vivo studies have shown that the high concentration of glucocorticoids can inhibit the proliferation of rat endogenous neural precursor cel s. Neuron-glia antigen 2 proteoglycan-positive neural progenitor cel s are the largest proliferating cel population in the mature central nervous system, which are pluripotent cel s. OBJECTIVE:To investigate the effects of glucocorticoid on the survival and proliferation of the neuron-glia antigen 2 proteoglycan-positive neural progenitor cel s from in vitro cultured adult rat hippocampus. METHODS:Neuron-glia antigen 2 proteoglycan-positive neural progenitor cel s were primary cultured and sub-cultured. The passage 1 cel s were treated with dexamethasone with the concentrations of 0, 0.1, 1, 10 and 100μmol for 48 hours, and then the cel activities were determined by lactate dehydrogenase assay, the apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay, and the proliferation of the cel s was identified by 5'-bromodeoxyuridine incorporation method. RESULTS AND CONCLUSION:Dexamethasone with the concentrations of 1, 10 and 100μmol could decrease the number of the neuron-glia antigen 2 proteoglycan-positive neural progenitor cel s, increase the number of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling positive cel s, and significantly reduce the number of BrdU positive cel s. High concentration of glucocorticoid can decrease the number of the neuron-glia antigen 2 proteoglycan-positive neural progenitor cel s by inhibiting the proliferation and inducing the apoptosis.