CAJ | 학술논문

背景:成纤维细胞生长因子2是神经系统主要的神经营养因子之一,目前已经被证明有促进内源性神经干细胞分化的作用.目的:观察成纤维细胞生长因子2干预下,肌萎缩侧索硬化SOD1G93A G1H转基因小鼠运动功能的改变,内源性神经干细胞的增殖情况,突触蛋白的水平改变及内源性神经干细胞增殖数目与突触蛋白水平改变的相关性.方法:取新出生 SOD1G93A G1H转基因小鼠(肌萎缩侧索硬化模型小鼠)60只分为肌萎缩侧索硬化组及成纤维细胞生长因子2组各30只,取新出生野生B6SJL小鼠30只作为正常对照组,成纤维细胞生长因子2组腹腔注入成纤维细胞生长因子2;肌萎缩侧索硬化组和正常对照组腹腔注入安慰剂生理盐水.分别于出生后60,90,120 d采用Rotarod方法评估小鼠运动功能的改变,采用免疫组织化学方法标记内源性神经干细胞和突触蛋白并计数,用spearman方法评估内源性神经干细胞增殖数目与突触蛋白水平的相关性.结果与结论:与肌萎缩侧索硬化组相比,成纤维细胞生长因子2组小鼠运动功能明显改善,内源性神经干细胞增殖和突触蛋白水平显著增高.小鼠内源性神经干细胞的增加与突触蛋白水平的增呈正相关.提示成纤维细胞生长因子2神经保护机制可能与其促进内源性神经干细胞增殖和提升突触蛋白水平相关.
배경:성섬유세포생장인자2시신경계통주요적신경영양인자지일,목전이경피증명유촉진내원성신경간세포분화적작용.목적:관찰성섬유세포생장인자2간예하,기위축측색경화SOD1G93A G1H전기인소서운동공능적개변,내원성신경간세포적증식정황,돌촉단백적수평개변급내원성신경간세포증식수목여돌촉단백수평개변적상관성.방법:취신출생 SOD1G93A G1H전기인소서(기위축측색경화모형소서)60지분위기위축측색경화조급성섬유세포생장인자2조각30지,취신출생야생B6SJL소서30지작위정상대조조,성섬유세포생장인자2조복강주입성섬유세포생장인자2;기위축측색경화조화정상대조조복강주입안위제생리염수.분별우출생후60,90,120 d채용Rotarod방법평고소서운동공능적개변,채용면역조직화학방법표기내원성신경간세포화돌촉단백병계수,용spearman방법평고내원성신경간세포증식수목여돌촉단백수평적상관성.결과여결론:여기위축측색경화조상비,성섬유세포생장인자2조소서운동공능명현개선,내원성신경간세포증식화돌촉단백수평현저증고.소서내원성신경간세포적증가여돌촉단백수평적증정정상관.제시성섬유세포생장인자2신경보호궤제가능여기촉진내원성신경간세포증식화제승돌촉단백수평상관.
BACKGROUND:Fibroblast growth factor 2 is one of the main neurotrophic factors for nervous system, has been proved to have a role in promoting the differentiation of endogenous neural stem cel s. OBJECTIVE:To detect the improvement of motor behavior and the proliferation of endogenous neural stem cel s, the level of synapsin and the correlation between the differentiation number of endogenous neural stem cel s and the level of synapsin in the SOD1G93A G1H transgenic mouse model of amyotrophic lateral sclerosis after fibroblast growth factor 2 administration. METHODS:Sixty new born SOD1G93A G1H transgenic mice (mouse models of amyotrophic lateral sclerosis) were divided into amyotrophic lateral sclerosis group and fibroblast growth factor 2 group, 30 mice in each group. Thirty newborn wild B6SJL mice were col ected as normal control group. Mice in the fibroblast growth factor 2 group received intraperitoneal injection of fibroblast growth factor 2;the mice in the amyotrophic lateral sclerosis group and the normal control group received intraperitoneal injection of placebo normal saline. The improvement of motor behavior was evaluated with Rotarod test at postnatal 60, 90 and 120 days;immunohistochemistry was used to mark the endogenous neural stem cel s and count the synapsin;the correlation between the differentiation number of endogenous neural stem cel s and the level of synapsin was evaluated with Spearman statistical method. RESULTS AND CONCLUSION:Compared with the amyotrophic lateral sclerosis group, the fibroblast growth factor 2 group showed a statistical y significant improvement in the motor behavior, number of endogenous neural stem cel s and level of synapsin were significantly increased. There was a negative correlation between the differentiation number of endogenous neural stem cel s and level of synapsin. The neuro-protection mechanism of fibroblast growth factor 2 may be associated with the proliferation of endogenous neural stem cel s and level of synapsin.