医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2013年
11期
17-18
,共2页
人参再造丸%小檗碱%反相高效液相色谱%含量测定%固相萃取
人參再造汍%小檗堿%反相高效液相色譜%含量測定%固相萃取
인삼재조환%소벽감%반상고효액상색보%함량측정%고상췌취
Renshenzaizaowan%Berberine%RP-HPLC%Quality control%Solid phase extract
目的:探讨用固相萃取法提取人参再造丸中总生物碱,并用 HPLC 测定小檗碱含量的方法.方法:用固相萃取小柱提取人参再造丸中的总生物碱,用HPLC法测定其中小檗碱的含量,色谱条件:以乙腈∶0.05mol/l KH2PO4缓冲液=28:72(V:V)为流动相,流速1.0ml/min;选用YMC-pack ODS-A (150×4.6mm,5μm)色谱柱;柱温40℃;紫外检测波长345nm;进样量10μl.结果:小檗碱的提取回收率为85.0%,回收率为101.0,HPLC 测定方法在浓度5~50μg/mL 范围内线性良好(r =0.9992),批内和批间 RSD=0.5%,1.0%.结论:本法样品处理简便、快速,杂质干扰小,测定结果准确可靠,也可用于人参再造丸中其他生物碱的定量分析.
目的:探討用固相萃取法提取人參再造汍中總生物堿,併用 HPLC 測定小檗堿含量的方法.方法:用固相萃取小柱提取人參再造汍中的總生物堿,用HPLC法測定其中小檗堿的含量,色譜條件:以乙腈∶0.05mol/l KH2PO4緩遲液=28:72(V:V)為流動相,流速1.0ml/min;選用YMC-pack ODS-A (150×4.6mm,5μm)色譜柱;柱溫40℃;紫外檢測波長345nm;進樣量10μl.結果:小檗堿的提取迴收率為85.0%,迴收率為101.0,HPLC 測定方法在濃度5~50μg/mL 範圍內線性良好(r =0.9992),批內和批間 RSD=0.5%,1.0%.結論:本法樣品處理簡便、快速,雜質榦擾小,測定結果準確可靠,也可用于人參再造汍中其他生物堿的定量分析.
목적:탐토용고상췌취법제취인삼재조환중총생물감,병용 HPLC 측정소벽감함량적방법.방법:용고상췌취소주제취인삼재조환중적총생물감,용HPLC법측정기중소벽감적함량,색보조건:이을정∶0.05mol/l KH2PO4완충액=28:72(V:V)위류동상,류속1.0ml/min;선용YMC-pack ODS-A (150×4.6mm,5μm)색보주;주온40℃;자외검측파장345nm;진양량10μl.결과:소벽감적제취회수솔위85.0%,회수솔위101.0,HPLC 측정방법재농도5~50μg/mL 범위내선성량호(r =0.9992),비내화비간 RSD=0.5%,1.0%.결론:본법양품처리간편、쾌속,잡질간우소,측정결과준학가고,야가용우인삼재조환중기타생물감적정량분석.
@@@@[ABSTRACT] OBJECTIVE: To improve determination methods of Berberines in Renshenzaizaowan. METHOD: Berberine and other Alkaloidin in Renshenzaizaowan were extracted by solid phase extract column from its diluted solution of methanol extraction and its content was determined by HPLC. The YMC-pack ODS-A C18 was used as separation column (150×4.6mm, 5μm) and the substance was eluted by mobile phase: V:V=acetonitrile:0.05mol/l KH2PO4=28:72, the flow rate was 1.0ml/min. DAD detector was used and detecting wave length was 345nm; the column temperature was 40℃ . RESULT: The linear range was 5 ~ 50 μg ( r = 9999). The average recovery of Berberine was 101.0 %. CONCLUSION: The method was rapid and simple, the peak disturbs slightly. The result was accurately and the method also applicable for quantitative analysis of other Alkaloid in Renshenzaizaowan.
