CAJ | 학술논문

目的建立舒肺糖浆的质量标准.方法采用薄层色谱法对处方中的甘草进行定性鉴别;采用高效液相色谱法对盐酸麻黄碱进行含量测定,色谱柱采用菲罗门C18(250mm×4.6mm,5μm),流动相为0.025mol/L磷酸二氢钠-甲醇(85:15),流速为1.0 ml/min,检测波长为210nm.结果在薄层色谱图中检出了甘草药材的特征斑点,阴性无干扰;盐酸麻黄碱进样量在0.032~0.795μg范围与峰面积线性关系良好,r=0.9997,平均回收率为99.54%(RSD=0.87%,n=6).结论该方法简便、准确、重复性好,可用于舒肺糖浆的质量控制.
목적건립서폐당장적질량표준.방법채용박층색보법대처방중적감초진행정성감별;채용고효액상색보법대염산마황감진행함량측정,색보주채용비라문C18(250mm×4.6mm,5μm),류동상위0.025mol/L린산이경납-갑순(85:15),류속위1.0 ml/min,검측파장위210nm.결과재박층색보도중검출료감초약재적특정반점,음성무간우;염산마황감진양량재0.032~0.795μg범위여봉면적선성관계량호,r=0.9997,평균회수솔위99.54%(RSD=0.87%,n=6).결론해방법간편、준학、중복성호,가용우서폐당장적질량공제.
Objective To establish the quality standard for Shufei Syrup. Methods Radix Glycyrrhizae was identified by TLC .Ephedrine HCl was determinated by HPLC,The Phenomenex C18 column(250 mm×4.6 mm,5μm)was used with the mobile phase of 0.025mol/L sodium dihydrogen phosphate anhydrous -methanol (85:15)at a flow rate of 1.0ml/min and the detection wavelength was set at 210nm. Results Radix Glycyrrhizae could be detected by TLC, negative determination without interference .Ephedrine HCl was linear within 0.032~0.795 g r=0.9997 and the average recovery rate was 99.54%(RSD=0.87%,n=6). Conclusion The established method is simple ,accurate and better repeatable,which can be used for the quality control of Shufei Syrup..