CAJ | 학술논문

目的研究自然杀伤T细胞(NK T细胞)在慢性生殖道炎症患者精液中的表达.方法筛选40例罹患生殖道炎症患者精液标本及20例正常精液标本,采用流式细胞术检测NK T细胞在患者精液中的表达.通过酶联免疫吸附试验在患者精浆检测炎性细胞因子:白介素6(IL-6),IL-17及γ-干扰素(IFN-γ).运用SPSS软件对NK T细胞百分率和精子密度、活力和存活率分析,同时对精液IL-6、IL-17以及IFN-γ的相关性进行研究.结果 NK T细胞在一部分慢性生殖道炎症患者中数目显著增高(n =20),而正常组则未能检测到其表达;NK T细胞数目与精子浓度、活力及存活率具有显著负相关性;在NK T细胞高表达组,NK T细胞数量与精浆IL-6、IFN-γ浓度具有显著相关性,但与精浆IL-17不具有显著相关性.结论在慢性生殖道炎症中NK T细胞增殖可伴随对精子的自身免疫反应,其通过释放IL-6、IFN-γ等影响精子质量.
목적연구자연살상T세포(NK T세포)재만성생식도염증환자정액중적표체.방법사선40례리환생식도염증환자정액표본급20례정상정액표본,채용류식세포술검측NK T세포재환자정액중적표체.통과매련면역흡부시험재환자정장검측염성세포인자:백개소6(IL-6),IL-17급γ-간우소(IFN-γ).운용SPSS연건대NK T세포백분솔화정자밀도、활력화존활솔분석,동시대정액IL-6、IL-17이급IFN-γ적상관성진행연구.결과 NK T세포재일부분만성생식도염증환자중수목현저증고(n =20),이정상조칙미능검측도기표체;NK T세포수목여정자농도、활력급존활솔구유현저부상관성;재NK T세포고표체조,NK T세포수량여정장IL-6、IFN-γ농도구유현저상관성,단여정장IL-17불구유현저상관성.결론재만성생식도염증중NK T세포증식가반수대정자적자신면역반응,기통과석방IL-6、IFN-γ등영향정자질량.
@@@@Objective To investigate the number of natural killer T cells (NK T cell) in semen of patients with chronic inflammation of genital tract. Methods Fresh semen from patients with chronic inflammation of genital tract (n=40) and healthy men (n = 20) were collected and analyzed according to the WHO criteria. The amount of NK T cells were detected by the invariant (Vα24-JαQ) TCR chain using flow cytometry. Levels of inflammatory cytokines interleukin-6 (IL-6), IL-17 and IFN-γ in cell-free seminal plasma were detected by an enzyme-linked immunosorbent assay (ELISA). The correlation between the percentage of NK T cells and spermatozoa concentration, motility, vitality, seminal IL-6, IL-17 as well as IFN-γ was investigated respectively by SPSS software. Results The percentages of NK T cells were increased significantly in some samples (n = 20), but not in all samples. A negative correlation was detected between the percentage of NK T cells and spermatozoa concentration, motility and vitality in high percentage group (n = 20). Interestingly, a significant correlation of iNK T cells to seminal IL-6, IFN-γ was detected whereas lack of correlation between iNK T cells and IL-17 in High group. Conclusion The proliferation of iNK T cells might produce an autoimmune response to spermatozoa and further affect sperm quality through secretion of IFN-γ not IL-17 under chronic inflammatory condition.