CAJ | 학술논문

将性成熟中华乌塘鳢(Bostrichthys sinensis)分别暴露于性信息素17α-P和PGE224 h和48 h后,检测其排卵率,并应用免疫细胞化学(SABC)法检测嗅觉系统上17α-P 受体(PR)和 PGE2受体(EPs)免疫阳性细胞数量的变化.结果发现暴露后雌鱼排卵率升高,且暴露48 h后的排卵率高于暴露24 h后的排卵率;嗅觉系统PR和EPs免疫阳性细胞数量增加.雌鱼排卵率的升高与PR和EPs免疫阳性细胞数量的增加具有一致性.研究结果提示,环境中的17α-P 和PGE2有可能通过中华乌塘鳢嗅觉系统受体PR和EPs的介导,影响其生殖状态.
장성성숙중화오당례(Bostrichthys sinensis)분별폭로우성신식소17α-P화PGE224 h화48 h후,검측기배란솔,병응용면역세포화학(SABC)법검측후각계통상17α-P 수체(PR)화 PGE2수체(EPs)면역양성세포수량적변화.결과발현폭로후자어배란솔승고,차폭로48 h후적배란솔고우폭로24 h후적배란솔;후각계통PR화EPs면역양성세포수량증가.자어배란솔적승고여PR화EPs면역양성세포수량적증가구유일치성.연구결과제시,배경중적17α-P 화PGE2유가능통과중화오당례후각계통수체PR화EPs적개도,영향기생식상태.
Our prior research suggests that 17α-P and PGE2 may act as putative sex pheromones in Chinese black sleeper (Bostrichthys sinensis), and that the pheromones are likely detected through the olfactory system by 17α-P receptors (PRs) and PGE2 receptors (EPs). During the spawning season, after mature B. sinensis were exposed to 17α-P or PGE2 for 24 h and 48 h, we measured the ovulation rates and quantified the immunoreactivities of PR and EPs in the olfactory system using SABC immunocytochemistry. The ovulation rates of females increased after exposure to 17α-P or PGE2 for 24 h and 48 h. Furthermore, the rate was the higher after 48 h exposure than after 24 h exposure. The increase in numbers of PR and EPs immunoreactive cells was the highest in the olfactory epi-thelium, followed by the olfactory bulb, and the olfactory nerve was the lowest exposed to 17α-P or PGE2. The increases in the number of PR and EPs immunoreactive cells were consistent with the increases in ovulation rate. Our results suggest that water-borne 17α-P and PGE2 affect the reproductive status of B. sinensis via the PRs and EPs in the olfactory system. We also discuss the potential mechanisms of sex pheromone reception and transduc-tion in the B. sinensis olfactory system.