CAJ | 학술논문

建立了采用 QuEChERS 试剂盒对烟叶样品进行提取、净化,采用 LC-MS/MS 测定烟草中57种有机氮、有机磷农药分析方法.将烟草样品用水浸润后加入乙腈振荡,QuEChERS 试剂盒提取、净化,以电喷雾正离子多反应检测方式(MRM)进行检测.结果显示57种农药在0.0003-0.5 mg/L 范围内线性良好,相关系数(r)在0.9935-0.9998,LOQ 为0.006-0.169 mg/kg (S/N=10),加标回收率为65.2%-103.8%,相对标准偏差(RSD)为1.9%-8.6%.
건립료채용 QuEChERS 시제합대연협양품진행제취、정화,채용 LC-MS/MS 측정연초중57충유궤담、유궤린농약분석방법.장연초양품용수침윤후가입을정진탕,QuEChERS 시제합제취、정화,이전분무정리자다반응검측방식(MRM)진행검측.결과현시57충농약재0.0003-0.5 mg/L 범위내선성량호,상관계수(r)재0.9935-0.9998,LOQ 위0.006-0.169 mg/kg (S/N=10),가표회수솔위65.2%-103.8%,상대표준편차(RSD)위1.9%-8.6%.
QuEChERS extraction and LC-MS/MS approach for the determination of 57 pesticide residues in tobacco leaves was developed. Pesticide residues in tobacco samples were extracted and purified by QuEChERS kits and separated by 3.5 μm column, followed by MRM (multiple reaction monitoring) test,and quantified by matrix-matched standard calibration. Results showed that the linear range was from 0.0003 to 0.5 mg/L. LOQ were 0.006~0.169 mg/kg (S/N=10), while average recovery rate ranged from 65.2% to 103.8% with relative standard deviations (RSD) from 1.9% to 8.6%.