中国医药指南
中國醫藥指南
중국의약지남
CHINA MEDICINE GUIDE
2013年
9期
62
,共1页
跌打巴布剂%高效液相色谱法%三七皂苷R1%人参皂苷Rg1
跌打巴佈劑%高效液相色譜法%三七皂苷R1%人參皂苷Rg1
질타파포제%고효액상색보법%삼칠조감R1%인삼조감Rg1
Dieda cataplasm%HPLC%Notoginsenoside R1%Ginsenoside Rg1
目的建立跌打巴布剂中三七皂苷R1和人参皂苷Rg1的含量测定方法.方法采用HPLC法,以Agilent TC-C18 ODS柱为色谱柱,以乙腈-水(24:76)为流动相,检测波长203nm,流速1mL/min.结果三七皂苷R1线性范围为0.583~29.15μg(r=0.9999,n=5),回收率为99.9%~100.3%.人参皂苷Rg1线性范围为0.336~16.8μg(r=0.9999,n=5),回收率为99.8%~100.2%.结论本方法准确、可靠、灵敏度高,可用于跌打巴布剂的定量检测.
目的建立跌打巴佈劑中三七皂苷R1和人參皂苷Rg1的含量測定方法.方法採用HPLC法,以Agilent TC-C18 ODS柱為色譜柱,以乙腈-水(24:76)為流動相,檢測波長203nm,流速1mL/min.結果三七皂苷R1線性範圍為0.583~29.15μg(r=0.9999,n=5),迴收率為99.9%~100.3%.人參皂苷Rg1線性範圍為0.336~16.8μg(r=0.9999,n=5),迴收率為99.8%~100.2%.結論本方法準確、可靠、靈敏度高,可用于跌打巴佈劑的定量檢測.
목적건립질타파포제중삼칠조감R1화인삼조감Rg1적함량측정방법.방법채용HPLC법,이Agilent TC-C18 ODS주위색보주,이을정-수(24:76)위류동상,검측파장203nm,류속1mL/min.결과삼칠조감R1선성범위위0.583~29.15μg(r=0.9999,n=5),회수솔위99.9%~100.3%.인삼조감Rg1선성범위위0.336~16.8μg(r=0.9999,n=5),회수솔위99.8%~100.2%.결론본방법준학、가고、령민도고,가용우질타파포제적정량검측.
Objective?Determine notoginsenoside R1 and ginsenoside Rg1 in Dieda cataplasm by HPLC. Methods Agilent TC-C18 ODS column was used with the mobile phase containing acetonitrile-water(24:76).The wavelength of detector was set at 203nm.The flow rate was 1mL/min. Results The calibration curves were linear in the range of 0.583~29.15μg for notoginsenoside R1(r=0.9999, n=5) and 0.336~16.8μg for ginsenoside Rg1(r=0.9999, n=5). The recovery of notoginsenoside R1 was 99.9%~100.3%, and that of ginsenoside Rg1 was 99.8%~100.2%. Conclusion The method is accurate, reliable and simple, and is suitable for the determination of Dieda cataplasm.
