中国医药指南
中國醫藥指南
중국의약지남
CHINA MEDICINE GUIDE
2013年
9期
404-406
,共3页
金松*%王瀚锐%刘彦东%范东旭%孙?瑶%苏德旺%柯德志%汪宝林
金鬆*%王瀚銳%劉彥東%範東旭%孫?瑤%囌德旺%柯德誌%汪寶林
금송*%왕한예%류언동%범동욱%손?요%소덕왕%가덕지%왕보림
血管内皮生长因子%碱性成纤维细胞生长因子%下肢动脉硬化闭塞%血管再生
血管內皮生長因子%堿性成纖維細胞生長因子%下肢動脈硬化閉塞%血管再生
혈관내피생장인자%감성성섬유세포생장인자%하지동맥경화폐새%혈관재생
Vascular endothelial growth factor, Basic fibroblast growth factor%Lower limb arteriosclerosis occlusive%Vascular regeneration
目的?探讨联合应用血管内皮生长因子(VEGF)与碱性成纤维细胞生长因子(bFGF)对大鼠后肢动脉硬化闭塞血管再生的作用机制.方法?建立大鼠后肢动脉硬化闭塞模型60只,随机分为4组,生理盐水治疗组(NS组)15只,碱性成纤维细胞生长因子组(bFGF组)15只,血管内皮生长因子组(VEGF组)15只,血管内皮生长因子+碱性成纤维细胞生长因子(VEGF+bFGF组)15只.NS组、VEGF组分别隔日一次腹腔注射生理盐水1mL、100μg/LVEGF?1mL;bFGF组隔日一次后肢股内侧多点肌肉注射100μg/L?bFGF?1mL;VEGF+bFGF组隔日一次腹腔注射100μg/L?LVEGF?1mL+后肢股内侧多点肌肉注射100μg/L??bFGF?1mL,共治疗21d.分别于7d、14d、21d观察大鼠后肢缺血情况如:间歇性跛行情况、皮肤颜色改变、皮温改变、足溃疡情况等.继续饲养至3个月后取后肢股内侧部位肌肉组织行免疫组化观察血管数.结果?VEGF+bFGF组微血管密度显著高于bFGF组、VEGF组及NS组,差异有显著性意义(P?<?0.05);其他各组间差异无显著性意义(P?>?0.05).可见VEGF与bFGF联合应用可促进后肢动脉硬化闭塞大鼠的血管再生.结论?VEGF联合bFGF能促进大鼠后肢动脉硬化闭塞血管再生,为临床治疗下肢动脉硬化闭塞提供新的治疗依据.
目的?探討聯閤應用血管內皮生長因子(VEGF)與堿性成纖維細胞生長因子(bFGF)對大鼠後肢動脈硬化閉塞血管再生的作用機製.方法?建立大鼠後肢動脈硬化閉塞模型60隻,隨機分為4組,生理鹽水治療組(NS組)15隻,堿性成纖維細胞生長因子組(bFGF組)15隻,血管內皮生長因子組(VEGF組)15隻,血管內皮生長因子+堿性成纖維細胞生長因子(VEGF+bFGF組)15隻.NS組、VEGF組分彆隔日一次腹腔註射生理鹽水1mL、100μg/LVEGF?1mL;bFGF組隔日一次後肢股內側多點肌肉註射100μg/L?bFGF?1mL;VEGF+bFGF組隔日一次腹腔註射100μg/L?LVEGF?1mL+後肢股內側多點肌肉註射100μg/L??bFGF?1mL,共治療21d.分彆于7d、14d、21d觀察大鼠後肢缺血情況如:間歇性跛行情況、皮膚顏色改變、皮溫改變、足潰瘍情況等.繼續飼養至3箇月後取後肢股內側部位肌肉組織行免疫組化觀察血管數.結果?VEGF+bFGF組微血管密度顯著高于bFGF組、VEGF組及NS組,差異有顯著性意義(P?<?0.05);其他各組間差異無顯著性意義(P?>?0.05).可見VEGF與bFGF聯閤應用可促進後肢動脈硬化閉塞大鼠的血管再生.結論?VEGF聯閤bFGF能促進大鼠後肢動脈硬化閉塞血管再生,為臨床治療下肢動脈硬化閉塞提供新的治療依據.
목적?탐토연합응용혈관내피생장인자(VEGF)여감성성섬유세포생장인자(bFGF)대대서후지동맥경화폐새혈관재생적작용궤제.방법?건립대서후지동맥경화폐새모형60지,수궤분위4조,생리염수치료조(NS조)15지,감성성섬유세포생장인자조(bFGF조)15지,혈관내피생장인자조(VEGF조)15지,혈관내피생장인자+감성성섬유세포생장인자(VEGF+bFGF조)15지.NS조、VEGF조분별격일일차복강주사생리염수1mL、100μg/LVEGF?1mL;bFGF조격일일차후지고내측다점기육주사100μg/L?bFGF?1mL;VEGF+bFGF조격일일차복강주사100μg/L?LVEGF?1mL+후지고내측다점기육주사100μg/L??bFGF?1mL,공치료21d.분별우7d、14d、21d관찰대서후지결혈정황여:간헐성파행정황、피부안색개변、피온개변、족궤양정황등.계속사양지3개월후취후지고내측부위기육조직행면역조화관찰혈관수.결과?VEGF+bFGF조미혈관밀도현저고우bFGF조、VEGF조급NS조,차이유현저성의의(P?<?0.05);기타각조간차이무현저성의의(P?>?0.05).가견VEGF여bFGF연합응용가촉진후지동맥경화폐새대서적혈관재생.결론?VEGF연합bFGF능촉진대서후지동맥경화폐새혈관재생,위림상치료하지동맥경화폐새제공신적치료의거.
Objective To study combined with application of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on rat hind?legs?arteriosclerosis?occlusive?vascular?regeneration?mechanism?of?action.?Methods?Model?rats?hind?limbs?arteriosclerosis?occlusive?60,?randomly?divided into 4 groups with physiological saline treatment group (NS) 15, of basic fibroblast growth factor (bFGF) 15, 15 group vascular endothelial growth factor (VEGF), vascular endothelial growth factor and basic fibroblast growth factor (VEGF+bFGF) 15. NS group, VEGF group respectively on a 1 mL celiac injection of saline, 100μg/LVEGF 1 mL;BFGF group on a hind legs of the medial more muscle injection 100μg/L bFGF 1 mL;Intraperitoneal injection of VEGF + bFGF group on a 100 μg/L LVEGF 1 mL+ hind legs of the medial more muscle injection 100 μg/L bFGF 1 mL, 21 days of treatment.?Respectively?in?7?days,?14,?21?days?observation?rat?hind?limbs?ischemia?conditions?such?as:?intermittent?claudication,?skin?color?change,?skin?temperature,?foot?ulcer.?Continue?to?raise?to?3?months?after?taking?hind?limbs?of?inside?part?muscle?line?immunohistochemical?observations?blood?vessels.?Results Microvascular density was significantly higher in VEGF+bFGF group than bFGF, VEGF and NS group, significant difference (P<0.05);?There?was?no significant difference between each other significance (P>0.05).?Visible?VEGF?and?bFGF?combination?can?promote?hind?legs?arteriosclerosis?occlusive?vascular?regeneration?in?rats.?Conclusion?VEGF?joint?bFGF?can?promote?the?rat?hind?legs?arteriosclerosis?occlusive?vascular?regeneration,?for?clinical?treatment?of?lower?limb?arteriosclerosis?occlusive?treatment?provides?a?new?basis.