CAJ | 학술논문

目的探究分析鼻咽癌咽后淋巴结转移与EBV-DNA血清学标记物的关系.方法选取2005年1月-2012年8月于东莞市人民医院治疗NPC患者共100例,测量NPC咽后转移淋巴结的横截面积,横截面积<5 mm为阴性,横截面积≥5 mm为阳性.利用PCR技术测定血清EBV-DNA水平,根据试剂盒给定的标准:>5×102 copies/mL为阳性,否则为阴性,即拷贝数为0.结果此100例患者发生咽后部淋巴结转移者共有88例(88.0%),无淋巴结转移者有22例(22.0%),其EBV-DNA含量水平为0 copies/mL.其中单侧咽后部淋巴结转移者有54例(54.0%),其EBV-DNA含量水平为2.2×104 copies/mL;双侧咽后部淋巴结转移者有34例(34.0%),其EBV-DNA含量水平最高为1.1×105 copies/mL.经统计学检验证明:阴性组与单侧阳性EBV-DNA含量水平以及单侧阳性组与双侧阳性组EBV-DNA含量水平比较,差异有统计学意义(P<0.05);结论 EBV-DNA的检测可以作为CNP患者的诊断依据之一,其含量亦可以对CNP是否发生咽喉淋巴道转移的判断对其病情的严重程度以及预后的判断以及有指导意义,值得临床推广及应用.
목적탐구분석비인암인후림파결전이여EBV-DNA혈청학표기물적관계.방법선취2005년1월-2012년8월우동완시인민의원치료NPC환자공100례,측량NPC인후전이림파결적횡절면적,횡절면적<5 mm위음성,횡절면적≥5 mm위양성.이용PCR기술측정혈청EBV-DNA수평,근거시제합급정적표준:>5×102 copies/mL위양성,부칙위음성,즉고패수위0.결과차100례환자발생인후부림파결전이자공유88례(88.0%),무림파결전이자유22례(22.0%),기EBV-DNA함량수평위0 copies/mL.기중단측인후부림파결전이자유54례(54.0%),기EBV-DNA함량수평위2.2×104 copies/mL;쌍측인후부림파결전이자유34례(34.0%),기EBV-DNA함량수평최고위1.1×105 copies/mL.경통계학검험증명:음성조여단측양성EBV-DNA함량수평이급단측양성조여쌍측양성조EBV-DNA함량수평비교,차이유통계학의의(P<0.05);결론 EBV-DNA적검측가이작위CNP환자적진단의거지일,기함량역가이대CNP시부발생인후림파도전이적판단대기병정적엄중정도이급예후적판단이급유지도의의,치득림상추엄급응용.
Objective To explore the analysis of nasopharyngeal carcinoma lymph node metastasis and serological markers of EBV-DNA Methods NPC patients from January 2005 to August 2012 a total of 100 cases.Measurement NPC retropharyngeal lymph node metastases the cross-sectional area, the cross-sectional area of<5 mm negative cross-sectional area of≥5 mm positive. The determination of the level of serum EBV-DNA, using PCR technology according to the the kit given standard:>5 × 102 copies/mL positive or negative, i.e., the copy number is 0. Results This 100 patients retropharyngeal lymph node metastasis, a total of 88 cases (88.0%) without lymph node metastasis in 22 patients (22.0%), the levels of EBV-DNA 0 copies/mL. Unilateral retropharyngeal lymph node metastasis in 54 patients (54.0%), the levels of EBV-DNA 2.2 × 104 copies/mL;bilateral retropharyngeal lymph node metastasis in 34 patients (34.0%), the EBV-DNA The concentration levels of up to 1.1 × 105 copies/mL. The statistical test proved:negative group compared with the levels of EBV-DNA positive group with bilateral and unilateral positive EBV-DNA content level as well as unilateral positive group difference was statistically significant (P<0.05);Conclusion EBV-DNA detection can be used as one of the CNP patients diagnosis is based on the content of CNP occurred throat lymph node metastasis to determine the severity of their illness and prognosis and guiding significance worthy of promotion and application.