CAJ | 학술논문

目的:研究槲皮素体外对人肝癌细胞凋亡及其凋亡通路的影响.方法:体外培养人肝癌SMMC-7721细胞,采用MTT法检测细胞增殖;采用流式细胞术检测凋亡率和细胞周期;应用ELISA法检测细胞上清液中Caspase-3、Caspase-9及Survivin的含量.结果:槲皮素(10-320μmol·L-1)体外可明显抑制SMMC-7721细胞的增殖,并具有浓度和时间依耐性;在40-160μmol·L-1浓度下,槲皮素可诱导SMMC-7721细胞凋亡;将细胞阻滞在G2-M期;使Caspase-3、Caspase-9蛋白表达量显著增加,而Survivin蛋白表达显著减少.结论:槲皮素抑制人肝癌SMMC-7721细胞的作用机制与诱导细胞凋亡和将细胞周期阻滞在G2-M期有关,其凋亡通路涉及Caspase途径.
목적:연구곡피소체외대인간암세포조망급기조망통로적영향.방법:체외배양인간암SMMC-7721세포,채용MTT법검측세포증식;채용류식세포술검측조망솔화세포주기;응용ELISA법검측세포상청액중Caspase-3、Caspase-9급Survivin적함량.결과:곡피소(10-320μmol·L-1)체외가명현억제SMMC-7721세포적증식,병구유농도화시간의내성;재40-160μmol·L-1농도하,곡피소가유도SMMC-7721세포조망;장세포조체재G2-M기;사Caspase-3、Caspase-9단백표체량현저증가,이Survivin단백표체현저감소.결론:곡피소억제인간암SMMC-7721세포적작용궤제여유도세포조망화장세포주기조체재G2-M기유관,기조망통로섭급Caspase도경.
Objective: To study the influence on apoptosis and apoptosis pathway in human hepatoma SMMC-7721 cells by quercetin. Methods: SMMC-7721 cells were cultured in vitro, and MTT was performed to observe the proliferation. Flow cytometry was utilized for measure the apoptosis and cell cycle. The expressions of Caspase-3, Caspase-9 and Survivin in cultured supernatant were detected by ELISA assay. Results: Quercetin could inhibit the proliferation of SMMC-7721 cells in vitro at 10 to 320 μmol·L-1, which was concentration- and time-dependent. At a dosage between 40 and 160 μmol·L-1 in cultured cells, the apoptosis rates was elevated, and cell cycle was blocked in G2-M phase. Quercetin was able to increase the expressions of Caspase-3 and Caspase-9 proteins but decrease the expression of Survivin protein. Conclusion: Quercetin can inhibit the proliferation of SMMC-7721 cells via the apoptosis induction and cell cycle inhibition, moreover, Caspase pathway was involved in the apoptosis pathway.