粮食与油脂
糧食與油脂
양식여유지
CEREALS & OILS
2013年
6期
50-52
,共3页
杜延兵%慕鸿雁%何忠宝%张自立%李苹苹
杜延兵%慕鴻雁%何忠寶%張自立%李蘋蘋
두연병%모홍안%하충보%장자립%리평평
大孔吸附树脂%葵花籽粕%绿原酸
大孔吸附樹脂%葵花籽粕%綠原痠
대공흡부수지%규화자박%록원산
macroporous adsorption resin%sunflower flake%chlorogenic acid
该实验研究D101大孔吸附树脂反相柱层析纯化绿原酸工艺;通过实验确定最佳条件为:洗脱剂为20%乙醇、洗脱剂用量为5 BV、上样量为1.5 BV、洗脱流速为2 ml/min;在此条件下,绿原酸产品纯度由原70.10%提高至90.41%,得率为64.23%.
該實驗研究D101大孔吸附樹脂反相柱層析純化綠原痠工藝;通過實驗確定最佳條件為:洗脫劑為20%乙醇、洗脫劑用量為5 BV、上樣量為1.5 BV、洗脫流速為2 ml/min;在此條件下,綠原痠產品純度由原70.10%提高至90.41%,得率為64.23%.
해실험연구D101대공흡부수지반상주층석순화록원산공예;통과실험학정최가조건위:세탈제위20%을순、세탈제용량위5 BV、상양량위1.5 BV、세탈류속위2 ml/min;재차조건하,록원산산품순도유원70.10%제고지90.41%,득솔위64.23%.
In this paper,the process of purifying Chlorogenic acid from sunflower flake with D101 Macroporous adsorption resin was studied,and finally confirmed the optimal eluant 20%ethanol,the volume of eluant 5BV,the volume of original solution 1.5 BV and the rate of flow 2 ml/min.The purity of final product was raised from 70.10%to 90.41%,and the yield was 64.23%.
