商丘师范学院学报
商丘師範學院學報
상구사범학원학보
JOURNAL OF SHANGQIU TEACHERS COLLEGE
2013年
6期
51-55
,共5页
卞杰松%冯纪南%谭巧燕%邓斌
卞傑鬆%馮紀南%譚巧燕%鄧斌
변걸송%풍기남%담교연%산빈
马鞭草%超声提取%总黄酮%抗氧化活性
馬鞭草%超聲提取%總黃酮%抗氧化活性
마편초%초성제취%총황동%항양화활성
Verbena officinalis L.%ultrasonic -assisted extraction%total flavonoids%antioxidant activity
采用正交试验设计研究超声波辅助提取马马鞭草中总黄酮的工艺条件,并对马鞭草中黄酮类化合物的抗氧化活进行测定.结果表明,马鞭草中总黄酮的最佳超声提取工艺条件为乙醇溶液体积分数70%、固液比1∶40(g/mL)、超声功率500 W、超声辅助提取温度70℃条件下提取20 min,在此条件下提取率可达2.87%.影响马鞭草中总黄酮提取效果的主次因素为:固液比>超声波功率>提取温度>乙醇体积分数.马鞭草黄酮类化合物具有清除羟自由基、超氧阴离子自由基的作用,其清除效果在一定范围内随着黄酮类化合物质量浓度的增加而增强.
採用正交試驗設計研究超聲波輔助提取馬馬鞭草中總黃酮的工藝條件,併對馬鞭草中黃酮類化閤物的抗氧化活進行測定.結果錶明,馬鞭草中總黃酮的最佳超聲提取工藝條件為乙醇溶液體積分數70%、固液比1∶40(g/mL)、超聲功率500 W、超聲輔助提取溫度70℃條件下提取20 min,在此條件下提取率可達2.87%.影響馬鞭草中總黃酮提取效果的主次因素為:固液比>超聲波功率>提取溫度>乙醇體積分數.馬鞭草黃酮類化閤物具有清除羥自由基、超氧陰離子自由基的作用,其清除效果在一定範圍內隨著黃酮類化閤物質量濃度的增加而增彊.
채용정교시험설계연구초성파보조제취마마편초중총황동적공예조건,병대마편초중황동류화합물적항양화활진행측정.결과표명,마편초중총황동적최가초성제취공예조건위을순용액체적분수70%、고액비1∶40(g/mL)、초성공솔500 W、초성보조제취온도70℃조건하제취20 min,재차조건하제취솔가체2.87%.영향마편초중총황동제취효과적주차인소위:고액비>초성파공솔>제취온도>을순체적분수.마편초황동류화합물구유청제간자유기、초양음리자자유기적작용,기청제효과재일정범위내수착황동류화합물질량농도적증가이증강.
@@@@The orthogonal experimental design method was employed to study the ultrasonic -assisted extraction process of total flavonoids from Verbena officinalis L.and their scavenging activities against hydroxyl and superoxide anion free radicals were evaluated.The optimum conditions for extracting total flavonoids from Verbena officinalis L. were found to be: ethanol concentration 70%, solid -to -liquid ratio 1∶40 (g/mL), ultrasonic power for 500 W, extraction temperature 70 ℃ and length of ultrasonic treatment of 20 min.Under these optimum conditions , the extraction efficiency of total flavonoids reached as high as 2.87%.Extraction temperature had the most important effect on the extraction of total flavnoids from Verbena officinalis L., followed by length of ultrasonic treatment, material -to -liquid ratio and ethanol concentration .The flavonoids extraction from Verbena officinalis L.had marked scavenging effects on both hydroxyl and superoxide anion free radicals in a concentration -dependent fashion.