潍坊医学院学报
濰坊醫學院學報
유방의학원학보
JOURNAL OF WEIFANG MEDICAL COLLEGE
2013年
3期
164-167
,共4页
赵瑶*%赵荣荣%祝令称%崔社娟%张仕状
趙瑤*%趙榮榮%祝令稱%崔社娟%張仕狀
조요*%조영영%축령칭%최사연%장사상
扩散加权成像%抗肿瘤%VX2移植瘤
擴散加權成像%抗腫瘤%VX2移植瘤
확산가권성상%항종류%VX2이식류
Diffusion-weighted Imaging%Antitumor%VX2 carcinoma
目的 探讨扩散加权成像(DWI)评价壁虎活性成分对兔VX2肝移植瘤疗效的价值,为壁虎活性成分的进一步研究提供实验依据.方法 用中药无蹼壁虎提取分离得到抗肿瘤活性成分,制成卵磷脂脂质体.建立兔VX2肝移植瘤模型,12d后肿瘤生长至0.5~1.5cm.将16只荷瘤兔随机分为活性成分组和生理盐水组,分别经耳缘静脉注射壁虎活性成分0.3mg/kg和0.9%生理盐水10ml/kg,每天1次,持续用药12d.于用药前1d及用药后第2,7,13天行STIR-EPI-DWI检查,分别测量各组肿瘤不同时间点的表观弥散系数(ADC)值.用药12d后处死全部荷瘤兔,肿瘤组织切片行TUNEL法检测肿瘤细胞的凋亡.结果 活性成分组用药前1d,第2,7,13天的ADC值分别为(1.13±0.09)×10-3 mm 2/s,(1.35±0.10)×10-3 mm 2/s,(1.62±0.12)×10-3 mm 2/s,(1.95±0.11)×10-3 mm 2/s,生理盐水组相应时间点的ADC值分别为(1.14±0.05)×10-3 mm 2/s,(1.15±0.04)×10-3 mm 2/s,(1.18±0.05)×10-3 mm 2/s,(1.26±0.06)×10-3 mm 2/s.除用药前1d外,活性成分组ADC值较生理盐水组增高并均有统计学意义(P<0.01). TUNEL法检测肿瘤细胞凋亡指数显示,对照组(4.70%±1.49%)与活性成分组(17.14%±1.67%)之间差异性具有统计学意义(P<0.01),且ADC值与凋亡指数AI呈明显正相关(r=0.800,P=0.000).结论 DWI可以准确、客观、无创地评价壁虎活性成分对兔VX2肝移植瘤的抑制作用.
目的 探討擴散加權成像(DWI)評價壁虎活性成分對兔VX2肝移植瘤療效的價值,為壁虎活性成分的進一步研究提供實驗依據.方法 用中藥無蹼壁虎提取分離得到抗腫瘤活性成分,製成卵燐脂脂質體.建立兔VX2肝移植瘤模型,12d後腫瘤生長至0.5~1.5cm.將16隻荷瘤兔隨機分為活性成分組和生理鹽水組,分彆經耳緣靜脈註射壁虎活性成分0.3mg/kg和0.9%生理鹽水10ml/kg,每天1次,持續用藥12d.于用藥前1d及用藥後第2,7,13天行STIR-EPI-DWI檢查,分彆測量各組腫瘤不同時間點的錶觀瀰散繫數(ADC)值.用藥12d後處死全部荷瘤兔,腫瘤組織切片行TUNEL法檢測腫瘤細胞的凋亡.結果 活性成分組用藥前1d,第2,7,13天的ADC值分彆為(1.13±0.09)×10-3 mm 2/s,(1.35±0.10)×10-3 mm 2/s,(1.62±0.12)×10-3 mm 2/s,(1.95±0.11)×10-3 mm 2/s,生理鹽水組相應時間點的ADC值分彆為(1.14±0.05)×10-3 mm 2/s,(1.15±0.04)×10-3 mm 2/s,(1.18±0.05)×10-3 mm 2/s,(1.26±0.06)×10-3 mm 2/s.除用藥前1d外,活性成分組ADC值較生理鹽水組增高併均有統計學意義(P<0.01). TUNEL法檢測腫瘤細胞凋亡指數顯示,對照組(4.70%±1.49%)與活性成分組(17.14%±1.67%)之間差異性具有統計學意義(P<0.01),且ADC值與凋亡指數AI呈明顯正相關(r=0.800,P=0.000).結論 DWI可以準確、客觀、無創地評價壁虎活性成分對兔VX2肝移植瘤的抑製作用.
목적 탐토확산가권성상(DWI)평개벽호활성성분대토VX2간이식류료효적개치,위벽호활성성분적진일보연구제공실험의거.방법 용중약무복벽호제취분리득도항종류활성성분,제성란린지지질체.건립토VX2간이식류모형,12d후종류생장지0.5~1.5cm.장16지하류토수궤분위활성성분조화생리염수조,분별경이연정맥주사벽호활성성분0.3mg/kg화0.9%생리염수10ml/kg,매천1차,지속용약12d.우용약전1d급용약후제2,7,13천행STIR-EPI-DWI검사,분별측량각조종류불동시간점적표관미산계수(ADC)치.용약12d후처사전부하류토,종류조직절편행TUNEL법검측종류세포적조망.결과 활성성분조용약전1d,제2,7,13천적ADC치분별위(1.13±0.09)×10-3 mm 2/s,(1.35±0.10)×10-3 mm 2/s,(1.62±0.12)×10-3 mm 2/s,(1.95±0.11)×10-3 mm 2/s,생리염수조상응시간점적ADC치분별위(1.14±0.05)×10-3 mm 2/s,(1.15±0.04)×10-3 mm 2/s,(1.18±0.05)×10-3 mm 2/s,(1.26±0.06)×10-3 mm 2/s.제용약전1d외,활성성분조ADC치교생리염수조증고병균유통계학의의(P<0.01). TUNEL법검측종류세포조망지수현시,대조조(4.70%±1.49%)여활성성분조(17.14%±1.67%)지간차이성구유통계학의의(P<0.01),차ADC치여조망지수AI정명현정상관(r=0.800,P=0.000).결론 DWI가이준학、객관、무창지평개벽호활성성분대토VX2간이식류적억제작용.
@@@@ Objective Investigate the diffusion-weighted imaging(DWI) value of rabbit VX2 hepatic tumor efficacy evaluation gecko active ingredient to provide experimental basis for further study of the active ingredient of gecko .Methods Antitumor activity with the herbs swinhonis gecko extraction and separation of components made of lecithin liposomes .Rabbit VX2 hepatic tumor model was established , and the tumor grew to 0.5~1.5cm after 12d.Sixteen tumor-bearing rabbits were randomly divided into the active ingredient group and saline group,respectively,by ear vein injection gecko active ingredient 0.3mg/kg and 0.9% saline 10ml/kg daily,continuous medication for 12 days.2,7,13-day line STIR,one day before treatment and after treatment-EPI-DWI check,and different time points of tumor apparent diffu-sion coefficient(apparent diffusion coefficient ,ADC) values were measured.Execution of all tumor-bearing rabbits was performed after 12d of medication,and tumor cell apoptosis were detected by TUNEL method .Results ADC values before 1 day of administration in the active in-gredient group and after 2,7,13 days were (1.13 ±0.09) ×10-3 mm 2/s,(1.35 ±0.10) ×10-3 mm 2/s,(1.62 ±0.12) ×10-3 mm 2/s and (1.95 ±0.11) ×10-3 mm 2/s respectively;and at the corresponding time points in saline group were (1.14 ±0.05) ×10-3 mm 2/s,(1.15 ± 0.04) ×10 -3 mm 2/s,(1.18 ±0.05) ×10-3 mm 2/s and (1.26 ±0.06) ×10-3 mm 2/s,respectively.Except 1 day before treatment,ADC val-ue of the active ingredient group increased compared with the saline group ,and the difference was statistically significant (P<0.01).TUNEL assayed apoptotic index,the difference between the control group (4.70%±1.49%) and the active ingredient group (17.14%±1.67%) was statistically significant(P<0.01),and ADC value and apoptosis index AI had significant positive correlation (r=0.800,P=0.000). Conclusion DWI can be accurate,objective and non-invasive evaluation of gecko active ingredients of rabbit VX 2 hepatic tumor inhibition.
