CAJ | 학술논문

目的提取中药北五味子中的多糖成分,制备多糖单一组分,并测定其表观分子量.方法采用沸水浸提法提取北五味子多糖,利用DEAE-cellulose52离子交换层析,Sephacryl S-200 HR凝胶层析技术对粗多糖进行纯化,得到单一组分.对所得单一组分多糖采用紫外光谱扫描和凝胶层析法进行纯度鉴定,并采用凝胶层析法测定多糖表观分子量.结果 100g北五味子提取得到粗多糖SCP5.71g,SCP经离子交换层析进一步分离后得到3个组分SCP-Ⅰ(提取率17.60%),SCP-Ⅱ(29.51%)和SCP-Ⅲ(6.30%),对主要组分SCP-Ⅰ和SCP-Ⅱ利用凝胶层析进一步纯化得到多糖单一组分SCP-Ⅰa和SCP-Ⅱa,凝胶层析法测定表观分子量分别为4.05kD和7.96kD.结论中药北五味子中主要还有两种多糖组分,SCP-Ⅰa,SCP-Ⅱa,它们的表观分子量分别为4.05kD和7.96kD.
목적 제취중약북오미자중적다당성분,제비다당단일조분,병측정기표관분자량.방법 채용비수침제법제취북오미자다당,이용DEAE-cellulose52리자교환층석,Sephacryl S-200 HR응효층석기술대조다당진행순화,득도단일조분.대소득단일조분다당채용자외광보소묘화응효층석법진행순도감정,병채용응효층석법측정다당표관분자량.결과 100g북오미자제취득도조다당SCP5.71g,SCP경리자교환층석진일보분리후득도3개조분SCP-Ⅰ(제취솔17.60%),SCP-Ⅱ(29.51%)화SCP-Ⅲ(6.30%),대주요조분SCP-Ⅰ화SCP-Ⅱ이용응효층석진일보순화득도다당단일조분SCP-Ⅰa화SCP-Ⅱa,응효층석법측정표관분자량분별위4.05kD화7.96kD.결론 중약북오미자중주요환유량충다당조분,SCP-Ⅰa,SCP-Ⅱa,타문적표관분자량분별위4.05kD화7.96kD.
@@@@ Objective To extract the polysaccharide of Schisandra chinensis (Turcz.),to prepare the single component of the polysaccharide,and to determine the apparent molecular weight .Methods Hot water extraction was made to extract the polysaccharide of Schisandra chinensis(Turcz.).Gel chromatography and ion exchange chromatography were made to purify the crude polysaccharide ,and a single component was obtained .UV spectroscopy and gel chromatography were made to determine the purity of the results of single component polysaccharide.Results 5.71g crude polysaccharide was extracted from 100g of schisandra.Three components of SCP ,SCP-Ⅰ,SCP-Ⅱand SCP-Ⅲwere separated after ion exchange chromatography .The yield rate was 17.6%,29.51% and 6.3% for each component.The single polysaccharide components SCP-Ⅰa and SCP-Ⅱa were purified by gel chromatography of SCP-Ⅰand SCP-Ⅱ.The apparent molecular weight for SCP-Ⅰa and SCP-Ⅱa was 4.05kD and 7.96kD.Conclusion The apparent molecular weights of SCP-Ⅰa and SCP-Ⅱa,the two main single component polysaccharide extracted from Fructus schisandra chinensis ,were 4.05kD and 7.96kD respectively.