CAJ | 학술논문

利用大庆碱性寒地黑土分离得到的一株葡萄球菌XY‐C ,采用含甲胺磷的液态培养基对该菌株代谢产酶.通过单因素确定该酶对韭菜中甲胺磷农药残留的降解工艺参数,以甲胺磷降解率作为评价指标.获得了粗酶液对韭菜中甲胺磷农药降解的最佳降解条件为:降解温度45℃,酶液添加量20mL ,分别喷洒3次,于45℃降解反应20min ,采用最优降解条件,粗酶液对韭菜中甲胺磷农药的实际降解率达到67.17±1.17%.
이용대경감성한지흑토분리득도적일주포도구균XY‐C ,채용함갑알린적액태배양기대해균주대사산매.통과단인소학정해매대구채중갑알린농약잔류적강해공예삼수,이갑알린강해솔작위평개지표.획득료조매액대구채중갑알린농약강해적최가강해조건위:강해온도45℃,매액첨가량20mL ,분별분쇄3차,우45℃강해반응20min ,채용최우강해조건,조매액대구채중갑알린농약적실제강해솔체도67.17±1.17%.
@@@@The research uses a Staphylococcus Rosehbach XY‐C separated in the alkaline cold black soil in Daqing .Liquid culture is used to make Staphylococcus Rosehbach XY‐C produce methamidophos dgrada‐tion enzyme .Degradation technological parameters of the coarse enzyme to methamidophos pesticide resi‐dues are determined according to single factor test .The technological parameters use degradation ratio of methamidophos as evaluation indicator .Based on the experimental data ,the optimum degradation condi‐tions of coarse enzyme to the biodegradation of methamidophos in chives are obtained .The optimum con‐ditions are :optimal temperature is 45℃ ,optimal enzyme dosage is 20mL ,spray three times respective and optimal reaction time is 20min .Degradation ratio of coarse enzyme to methamidophos pesticide resi‐dues was 67 .17 ± 1 .17% based on the optimum degradation conditions .