中国林副特产
中國林副特產
중국림부특산
QUARTERLY OF FOREST BY-PRODUCT AND SPECIALITY IN CHINA
2013年
3期
32-35
,共4页
冯雪%张曜武*%曹炳星%王薇薇
馮雪%張曜武*%曹炳星%王薇薇
풍설%장요무*%조병성%왕미미
红松%多糖%含量测定%DNS法
紅鬆%多糖%含量測定%DNS法
홍송%다당%함량측정%DNS법
Pinus koraiensis%Polysaccharids%Content determination%3 ,5‐dinitrosalicylic acid col‐orimetry
为建立红松松塔多糖含量测定方法.应用DNS法分别测定还原糖及水解后总糖的含量,计算得出多糖含量.结果表明,以葡萄糖为对照品,此方法在20~67mg/L浓度范围内线性关系良好,回归方程A=18.7C-0.2232(R2=0.9993),平均加样回收率99.47%,RSD=1.68%.此法稳定性、重现性、精密度等均在规定范围内,过程简单,操作简便,可快速得出结果.
為建立紅鬆鬆塔多糖含量測定方法.應用DNS法分彆測定還原糖及水解後總糖的含量,計算得齣多糖含量.結果錶明,以葡萄糖為對照品,此方法在20~67mg/L濃度範圍內線性關繫良好,迴歸方程A=18.7C-0.2232(R2=0.9993),平均加樣迴收率99.47%,RSD=1.68%.此法穩定性、重現性、精密度等均在規定範圍內,過程簡單,操作簡便,可快速得齣結果.
위건립홍송송탑다당함량측정방법.응용DNS법분별측정환원당급수해후총당적함량,계산득출다당함량.결과표명,이포도당위대조품,차방법재20~67mg/L농도범위내선성관계량호,회귀방정A=18.7C-0.2232(R2=0.9993),평균가양회수솔99.47%,RSD=1.68%.차법은정성、중현성、정밀도등균재규정범위내,과정간단,조작간편,가쾌속득출결과.
@@@@Objective To establish a method for the determination of polysaccharides in Pinus ko‐raiensis corns .Methods Determine the content of reducing sugar and total sugar by DNS Meth‐od .Results Contrast to glucose ,the linear range was 20‐67mg/L (R 2 =0 .999 3) ,the average re‐covery was 99 .47% ,RSD=1 .68% .Conclusion The method is sensitive ,steady with good repeat‐ability .
