CAJ | 학술논문

以12份不同基因型的芥菜栽培种为试材,用NLN-13培养基进行游离小孢子培养,并进行不同的热激诱导处理,研究基因型、热激条件以及供体植株生长条件(主要是温度)对芥菜游离小孢子培养的影响.结果表明:芥菜小孢子培养的适宜花蕾长度为2~3 mm,此时小孢子主要处于单核靠边期,显微观察小孢子有3条明显的萌发沟;35℃热激处理3 d有利于芥菜小孢子诱导成胚;基因型是影响芥菜小孢子培养的关键因素,不同基因型芥菜的出胚能力差异很大,供试12份材料中有9份诱导出胚,其中V03B0097和A12959出胚率最高,分别达5.87胚·蕾-1和5.54胚·蕾-1;供体植株在较低的温度环境下生长有利于芥菜小孢子的胚胎发生.
이12빈불동기인형적개채재배충위시재,용NLN-13배양기진행유리소포자배양,병진행불동적열격유도처리,연구기인형、열격조건이급공체식주생장조건(주요시온도)대개채유리소포자배양적영향.결과표명:개채소포자배양적괄의화뢰장도위2~3 mm,차시소포자주요처우단핵고변기,현미관찰소포자유3조명현적맹발구;35℃열격처리3 d유리우개채소포자유도성배;기인형시영향개채소포자배양적관건인소,불동기인형개채적출배능력차이흔대,공시12빈재료중유9빈유도출배,기중V03B0097화A12959출배솔최고,분별체5.87배·뢰-1화5.54배·뢰-1;공체식주재교저적온도배경하생장유리우개채소포자적배태발생.
In order to improve the microspore culture system in Brassica juncea(L.) Czern. et Coss. and promote its application in breeding and basic research,we used 12 different genotypes of Brassica juncea (L.) Czern. et Coss. as experimental materials to study the effects of genotype,heat shock induction and donor plants growth conditions,especially atmospheric temperature on microspore culture in Brassica juncea(L.) Czern. et Coss.The results indicated that microspores in the late uninucleate stage were prismatoidal and characterized with 3 obvious germinal furrows,which can help identify the length of buds that contained a large proportion of late-uninucleate-stage microspores.2-3 mm buds were appropriate for microspore culture in Brassica juncea(L.)Czern. et Coss.35 °C heat shock for 3 days contributed to the induction of microspore embryogenesis. Genotype was the key factor that affected the results of microspore culture in Brassica juncea(L.)Czern. et Coss.The ability of embryo yield was quite different among 12 different genotypes.Nine of them produced embryos, V03B0097 and A12959 gave the highest among the 12 genotypes:5.87 and 5.54 embryos per bud, respectively.Low temperature for donor plants growing was appropriate for embryogenesis of microspore culture in Brassica juncea(L.)Czern. et Coss.