CAJ | 학술논문

为了探讨烟粉虱(Bemisia tabaci)血淋巴对白僵菌(Beauveria bassiana)的防御机制,利用吉姆萨染色法在光学显微镜下观察烟粉虱3龄若虫血细胞感染白僵菌后的免疫反应.结果表明:白僵菌的入侵可导致烟粉虱血细胞数量发生改变.体表接种36 h后,虫体内出现芽生孢子,血细胞数量开始增加,芽生孢子被吸附到浆血细胞表面;接种60 h后菌丝段吸附到粒血细胞表面;接种96 h后粒血细胞表面吸附大量菌丝段,此时昆虫体色已经呈现感染症状,说明真菌已经打破了烟粉虱的防御体系,烟粉虱幼虫血淋巴对白僵菌有短时间抑制.
위료탐토연분슬(Bemisia tabaci)혈림파대백강균(Beauveria bassiana)적방어궤제,이용길모살염색법재광학현미경하관찰연분슬3령약충혈세포감염백강균후적면역반응.결과표명:백강균적입침가도치연분슬혈세포수량발생개변.체표접충36 h후,충체내출현아생포자,혈세포수량개시증가,아생포자피흡부도장혈세포표면;접충60 h후균사단흡부도립혈세포표면;접충96 h후립혈세포표면흡부대량균사단,차시곤충체색이경정현감염증상,설명진균이경타파료연분슬적방어체계,연분슬유충혈림파대백강균유단시간억제.
To identify the defense mechanism of Bemisia tabaci hemolymph against Beauveria bassiana,cellular immune responses of the infected 3rd instar nymph was investigated with optics microscope and Giemsa stain.The results showed that the invasion of Beauveria bassiana could bring about changes in the total numbers of host hemocytes.After 36 hours inoculation,the blastospores appeared in B. tabaci and the total numbers of host hemocytes were increased.At the same time, the blastospores were adsorbed to the surface of plamatocyte.60 hours after inoculation,the hyphal bodies were absorbed to the surface of granulocytes.More hyphal bodies were observed to the surface of granulocytes 96 hours after inoculation,and the insect body color had appeared the symptoms of infection,which means that the B. bassiana has broken the defense of B. tabaci.B. tabaci can only inhibit the invasion of B. basiana temporarily.