CAJ | 학술논문

δ阿片受体激动剂DADLE对急性全脑缺血再灌注大鼠脑组织Bcl-2、Caspase-3的影响
δ아편수체격동제DADLE대급성전뇌결혈재관주대서뇌조직Bcl-2、Caspase-3적영향
Effects of DADLE on Bcl-2 and Caspase-3 of rats’ brain expression following cerebral- ischemia reperfusion injury

万方数据

中国医疗前沿中國醫療前沿 중국의료전연
CHINA HEALTHCARE INNOVATION
2013年 9期 21-23 ,共3页

文锐玲%郭伟%黄伟青%武钊%刘佩仪%洪妙玲%郭兰英%黄瑞娇%欧阳斌%梁庆

문예령%곽위%황위청%무쇠%류패의%홍묘령%곽란영%황서교%구양빈%량경

全脑缺血再灌注%DADLE%神经保护%Bcl-2%Caspase-3 全腦缺血再灌註%DADLE%神經保護%Bcl-2%Caspase-3
전뇌결혈재관주%DADLE%신경보호%Bcl-2%Caspase-3
Cerebral-ischemia reperfusion%Delta opioid peptide[d-Ala2, d-Leu5] enkephalin (DADLE)%Neuroprotection%Bcl-2%Caspase-3

目的研究全脑缺血再灌注给予δ阿片受体激动剂DADLE前后大鼠脑组织Bcl-2、Caspase-3的表达情况,探讨DADLE在全脑缺血再灌注损伤中对Bcl-2、Caspase-3的影响及与脑保护作用的关系.方法健康雌性SD大鼠50只(180-220g)随机分为5组:假手术组(Sham,n=10):只暴露血管而不夹闭;缺血再灌注组(I/R,n=10):采用改良的二血管阻断加低血压法建立全脑缺血再灌注模型,缺血15min后给予再灌注;DADLE处理组分为2mg/kg组(A,n=10)、DADLE 3mg/kg组(B,n=10)、DADLE 5mg/kg组(C,n=10):在I/R组的基础上于再灌注前经颈外静脉注射DADLE.各组动物再灌注120min后处死,开颅取大脑组织,采用western-bolt技术检测大鼠脑组织Bcl-2、Caspase-3的表达状况.结果 western-bolt检测结果显示,sham组Bcl-2表达水平显著低于其他各组(P<0.05),且Sham组仅少量Caspase-3表达.DADLE处理组与I/R组相比, Bcl-2表达水平上调(P<0.05)而Caspase-3表达水平下调(P<0.05).各给药组之间Bcl-2为B组与C组的表达显著高于A组(P<0.05),B组与C组间差异无统计学意义(P>0.05);Caspase-3表达水平为B组与C组的表达显著低于A组(P<0.05),而B组与C组间差异无统计学意义(P>0.05).结论药物DADLE可能通过上调Bcl-2的表达并抑制Caspase-3的表达,抑制神经细胞的凋亡,减低全脑缺血再灌注损伤,从而对大脑起保护作用.
목적연구전뇌결혈재관주급여δ아편수체격동제DADLE전후대서뇌조직Bcl-2、Caspase-3적표체정황,탐토DADLE재전뇌결혈재관주손상중대Bcl-2、Caspase-3적영향급여뇌보호작용적관계.방법건강자성SD대서50지(180-220g)수궤분위5조:가수술조(Sham,n=10):지폭로혈관이불협폐;결혈재관주조(I/R,n=10):채용개량적이혈관조단가저혈압법건립전뇌결혈재관주모형,결혈15min후급여재관주;DADLE처리조분위2mg/kg조(A,n=10)、DADLE 3mg/kg조(B,n=10)、DADLE 5mg/kg조(C,n=10):재I/R조적기출상우재관주전경경외정맥주사DADLE.각조동물재관주120min후처사,개로취대뇌조직,채용western-bolt기술검측대서뇌조직Bcl-2、Caspase-3적표체상황.결과 western-bolt검측결과현시,sham조Bcl-2표체수평현저저우기타각조(P<0.05),차Sham조부소량Caspase-3표체.DADLE처리조여I/R조상비, Bcl-2표체수평상조(P<0.05)이Caspase-3표체수평하조(P<0.05).각급약조지간Bcl-2위B조여C조적표체현저고우A조(P<0.05),B조여C조간차이무통계학의의(P>0.05);Caspase-3표체수평위B조여C조적표체현저저우A조(P<0.05),이B조여C조간차이무통계학의의(P>0.05).결론약물DADLE가능통과상조Bcl-2적표체병억제Caspase-3적표체,억제신경세포적조망,감저전뇌결혈재관주손상,종이대대뇌기보호작용.
@@@@Objective To determine expression of Bcl-2 and Caspase-3 in brain tissue of rat before and after using DADLE, and to investigate effects of DADLE on Bcl-2 and Caspase-3 expression following global cerebral- ischemia reperfusion injury and the relationship with Brain protection. Methods 50 Sprague-Daw-ley(SD) rats were randomly entolled into 5 groups: the sham-operated: only exposure blood vessels; the ischemia reperfusion group(I/R, n=10):improved establishment of two vessel occlusion add hypotensionand establish global cerebral-ischemia reperfusion, 15min after ischemia and give reperfusion; three DADLE-treated(different drug doses: 2mg/kg[A], 3mg/kg[B] and 5mg/kg[C]):via external jugular vein injection of DADLE before reperfusion on the basis of the I/R group. Each animal Put to death after reperfusion for 120min, Craniotomy for brain tissue, Using western-bolt technique to detect the expression of Caspase-3 and Bcl-2 in brain tissue of rat. Results Western-bolt test results: The expression level of Bcl-2 in sham group was significantly lower than that of other groups(P<0.05), and only a small amount of Caspase-3 expression in Sham group. DADLE group compared with I/R group, Bcl-2 expression level increased(P<0.05) and Caspase-3 expression level decreased(P<0.05). Each group of Bcl-2 between B group and C group expression was higher than that in A group(P<0.05), B group and C group had no difference(P>0.05); the expression level of Caspase-3 in B group and C group was significantly lower than that in group A expression(P<0.05), B group and C group differences no statistical significance(P>0.05). Conclusion Substance DADLE may through the up-regulation of Bcl-2 expression and inhibit the expression of Caspase-3, inhibit the apoptosis of nerve cells, reduce the total cerebral ischemia reperfusion injury, which helps protect the brain.