中国有色金属学报
中國有色金屬學報
중국유색금속학보
THE CHINESE JOURNAL OF NONFERROUS METALS
2013年
5期
1345-1350
,共6页
黎媛萍%曾光明%汤琳%陈耀宁%章毅%刘灿%李贞%庞娅
黎媛萍%曾光明%湯琳%陳耀寧%章毅%劉燦%李貞%龐婭
려원평%증광명%탕림%진요저%장의%류찬%리정%방아
修饰金电极%自组装单分子膜%基因%竞争杂交%电化学检测
脩飾金電極%自組裝單分子膜%基因%競爭雜交%電化學檢測
수식금전겁%자조장단분자막%기인%경쟁잡교%전화학검측
modified gold electrode%self-assembled monolayer%gene%competitive hybridization%electrochemical determination
采用分子自组装技术制备巯基修饰的基因分子膜修饰金电极,用于竞争式杂交检测黄孢原毛平革菌木素过氧化物酶编码基因.通过差分脉冲伏安法、循环伏安法、交流阻抗法和电流—时间曲线法优化自组装时间和信号探针的最佳响应浓度,研究目标基因的线性检测范围和再生性能.结果表明:修饰金电极最优自组装时间为15 h,信号探针的最佳响应浓度为0.51×10?6 mol/L,目标基因的线性检测范围为7.51×10?12~1.05×10?9 mol/L,检测下限为7.51×10?13 mol/L.该修饰电极具有良好的再生性能.
採用分子自組裝技術製備巰基脩飾的基因分子膜脩飾金電極,用于競爭式雜交檢測黃孢原毛平革菌木素過氧化物酶編碼基因.通過差分脈遲伏安法、循環伏安法、交流阻抗法和電流—時間麯線法優化自組裝時間和信號探針的最佳響應濃度,研究目標基因的線性檢測範圍和再生性能.結果錶明:脩飾金電極最優自組裝時間為15 h,信號探針的最佳響應濃度為0.51×10?6 mol/L,目標基因的線性檢測範圍為7.51×10?12~1.05×10?9 mol/L,檢測下限為7.51×10?13 mol/L.該脩飾電極具有良好的再生性能.
채용분자자조장기술제비구기수식적기인분자막수식금전겁,용우경쟁식잡교검측황포원모평혁균목소과양화물매편마기인.통과차분맥충복안법、순배복안법、교류조항법화전류—시간곡선법우화자조장시간화신호탐침적최가향응농도,연구목표기인적선성검측범위화재생성능.결과표명:수식금전겁최우자조장시간위15 h,신호탐침적최가향응농도위0.51×10?6 mol/L,목표기인적선성검측범위위7.51×10?12~1.05×10?9 mol/L,검측하한위7.51×10?13 mol/L.해수식전겁구유량호적재생성능.
A gold electrode with thiolated capture probe through self-assembling based on the competitive hybridization for detection of target sequence of lignin peroxidase (lip) gene of Phanerochaete chrysosporium was developed. Following hybridizations with competitively hybridized with the target nucleic acid and biotinylated response probe, streptavidin-horseradish peroxidase (HRP) conjugate was applied to the electrode. The electrochemical behavior was analyzed by cyclic voltammetry, electrochemical impedance spectroscopy, current—time curve and differential pulse voltammetry. The results show that the best time for self-assembling is 15 h, and the optimum concentration of response probe is 0.51×10?6 mol/L. A good linear correlation between the current and the concentration of the target nucleic acid concentration is found in the concentration range from 7.51×10?12 mol/L to 1.05×10?9 mol/L. The detection limit is 7.51×10?13 mol/L. The modified electrode exhibits high sensitivity, precision, stability and reproducibility.