中国有色金属学报
中國有色金屬學報
중국유색금속학보
THE CHINESE JOURNAL OF NONFERROUS METALS
2013年
5期
1447-1456
,共10页
钟婵娟%孙德四%王化军%张强
鐘嬋娟%孫德四%王化軍%張彊
종선연%손덕사%왕화군%장강
胶质芽孢杆菌%环状芽孢杆菌%亚硝酸钠%诱变%细菌浸出%脱硅%铝土矿
膠質芽孢桿菌%環狀芽孢桿菌%亞硝痠鈉%誘變%細菌浸齣%脫硅%鋁土礦
효질아포간균%배상아포간균%아초산납%유변%세균침출%탈규%려토광
Bacillus mucilaginosus%Bacillus circulans%sodium nitrite%mutagenesis%bioleaching%desilicon%bauxite
以胶质芽孢杆菌CGMCC11和环状芽孢杆菌CGMCC12为出发菌株,采用亚硝酸钠对其进行诱变育种与浸矿研究.结果表明:菌株CGMCC11和CGMCC12的最适生长温度分别为28和30℃,最适pH值分别为7.2和8.3.采用40和60 mg/L亚硝酸钠分别处理出发菌株CGMCC11和CGMCC12,致死率分别为87%和85%,正突变率分别为18%和20%;筛选获得两株突变菌CGMCC11KP和CGMCC12KP,其达到生长稳定期的时间分别比对应的出发菌株达到生长稳定期的时间缩短了48和24 h,且具有更大的菌体密度、产酸和产大分子胞外聚合物的能力.浸矿15 d,与对应的出发菌株相比,突变菌株CGMCC11KP和CGMCC12KP溶出的SiO2量分别提高了30.47%和29.57%,且达到浸出终点的时间分别提前了5和3 d;混合诱变菌株浸出液中SiO2的量分别比对应的诱变菌株浸出液中 SiO2的量提高了20.0%和37.5%,且达到浸出终点的时间比出发菌株达到浸出终点的时间提前了6 d.SEM和XRD结果表明:混合诱变菌株对铝土矿的溶蚀分解最为明显,混合浸出15 d后,诱变前后胶质芽孢杆菌CGMCC11和环状芽孢杆菌CGMCC12的菌落个数比由1:1变为10:1.
以膠質芽孢桿菌CGMCC11和環狀芽孢桿菌CGMCC12為齣髮菌株,採用亞硝痠鈉對其進行誘變育種與浸礦研究.結果錶明:菌株CGMCC11和CGMCC12的最適生長溫度分彆為28和30℃,最適pH值分彆為7.2和8.3.採用40和60 mg/L亞硝痠鈉分彆處理齣髮菌株CGMCC11和CGMCC12,緻死率分彆為87%和85%,正突變率分彆為18%和20%;篩選穫得兩株突變菌CGMCC11KP和CGMCC12KP,其達到生長穩定期的時間分彆比對應的齣髮菌株達到生長穩定期的時間縮短瞭48和24 h,且具有更大的菌體密度、產痠和產大分子胞外聚閤物的能力.浸礦15 d,與對應的齣髮菌株相比,突變菌株CGMCC11KP和CGMCC12KP溶齣的SiO2量分彆提高瞭30.47%和29.57%,且達到浸齣終點的時間分彆提前瞭5和3 d;混閤誘變菌株浸齣液中SiO2的量分彆比對應的誘變菌株浸齣液中 SiO2的量提高瞭20.0%和37.5%,且達到浸齣終點的時間比齣髮菌株達到浸齣終點的時間提前瞭6 d.SEM和XRD結果錶明:混閤誘變菌株對鋁土礦的溶蝕分解最為明顯,混閤浸齣15 d後,誘變前後膠質芽孢桿菌CGMCC11和環狀芽孢桿菌CGMCC12的菌落箇數比由1:1變為10:1.
이효질아포간균CGMCC11화배상아포간균CGMCC12위출발균주,채용아초산납대기진행유변육충여침광연구.결과표명:균주CGMCC11화CGMCC12적최괄생장온도분별위28화30℃,최괄pH치분별위7.2화8.3.채용40화60 mg/L아초산납분별처리출발균주CGMCC11화CGMCC12,치사솔분별위87%화85%,정돌변솔분별위18%화20%;사선획득량주돌변균CGMCC11KP화CGMCC12KP,기체도생장은정기적시간분별비대응적출발균주체도생장은정기적시간축단료48화24 h,차구유경대적균체밀도、산산화산대분자포외취합물적능력.침광15 d,여대응적출발균주상비,돌변균주CGMCC11KP화CGMCC12KP용출적SiO2량분별제고료30.47%화29.57%,차체도침출종점적시간분별제전료5화3 d;혼합유변균주침출액중SiO2적량분별비대응적유변균주침출액중 SiO2적량제고료20.0%화37.5%,차체도침출종점적시간비출발균주체도침출종점적시간제전료6 d.SEM화XRD결과표명:혼합유변균주대려토광적용식분해최위명현,혼합침출15 d후,유변전후효질아포간균CGMCC11화배상아포간균CGMCC12적균락개수비유1:1변위10:1.
The original strains, Bacillus mucilaginosus CGMCC11 and Bacillus circulans CGMCC12, were treated by sodium nitrite to induce mutagenesis and investigate bioleaching of bauxite. The results show that the optimum temperature and pH value are 28℃and 7.2 for B?M CGMCC11, and 30℃and 8.3 for B?C CGMCC12. The lethal rates of B?M CGMCC11 and B?C CGMCC12 are 87% and 85%, and the positive mutant rates are 18%and 20%after being treated by 40 and 60 mg/L sodium nitrite culturing, respectively. The two mutants, B?M CGMCC11KP and B?C CGMCC12KP, screened from positive mutant strains reach stationary stages by 48 and 24 h ahead of the corresponding original strains, and have higher bacterial concentrations and higher ability of producing organic acids and macromolecule extracellular polymers than the original strains. After leaching for 15 d, the SiO2 concentrations in supernatants of the mutant stains of B?M CGMCC11KP and B?C CGMCC12KP are improved by 30.47% and 29.57%, and the leaching time of 5 and 3 d shorter, respectively, than those of the two corresponding original strains. After bioleaching with the mixture of B?M CGMCC11KP and B?C CGMCC12KP, the SiO2 concentration of the supernatant is 20.0%and 37.5%higher than those of the corresponding mutants, and the mixture can shorten the leaching time by 6 d in comparison with the original strains. The SEM and XRD analyses of bauxite surfaces before and after leaching show that the mixture of B?M CGMCC11KP and B?C CGMCC12KP has the greatest corrosion and decomposition ability on bauxite. After bioleaching for 15 d, the proportion of cell density in the supernatants of B?M CGMCC11 and B?C CGMCC12 is changed from 1:1 to approximately 10:1.
