CAJ | 학술논문

目的制备胰岛素样生长因子-I明胶微球(IGF-I-GMs),观察其一般性质、体外释药性及对兔骨髓基质干细胞(BMSCs)增值和分化效应.方法采用乳化交联法制备IGF-I-GMs,观察其性质;ELISA试剂盒测定IGF-I的含量,计算微球包封率、载药率以及体外释药性.体外分离培养兔BMSCs,四唑盐比色法(MTT)检测IGF-I和IGF-I-GMs对BMSCs增值的影响.用PNPP偶氮法检测碱性磷酸酶活性,反应IGF-I和IGF-I-GMs对BMSCs向成骨细胞定向分化影响.结果①所制备的微球表面光滑圆整、均匀分布,微球载药量和包封率高,体外药物缓释作用好;②IGF-I和IGF-I-GMs均促进BMSCs增值,且IGF-I-GMs效果更加显著;③IGF-I和IGF-I-GMs均促进BMSCs向成骨细胞分化,且IGF-I-GMs效果更加显著.结论 IGF-I-GMs在7天内对IGF-I有良好的缓慢释放作用;BMSCs形态和活性良好;IGF-I-GMs与BMSCs有良好的生物相容性,且促进BMSCs增值及成骨作用均明显优于单独使用IGF-I.
목적제비이도소양생장인자-I명효미구(IGF-I-GMs),관찰기일반성질、체외석약성급대토골수기질간세포(BMSCs)증치화분화효응.방법채용유화교련법제비IGF-I-GMs,관찰기성질;ELISA시제합측정IGF-I적함량,계산미구포봉솔、재약솔이급체외석약성.체외분리배양토BMSCs,사서염비색법(MTT)검측IGF-I화IGF-I-GMs대BMSCs증치적영향.용PNPP우담법검측감성린산매활성,반응IGF-I화IGF-I-GMs대BMSCs향성골세포정향분화영향.결과①소제비적미구표면광활원정、균균분포,미구재약량화포봉솔고,체외약물완석작용호;②IGF-I화IGF-I-GMs균촉진BMSCs증치,차IGF-I-GMs효과경가현저;③IGF-I화IGF-I-GMs균촉진BMSCs향성골세포분화,차IGF-I-GMs효과경가현저.결론 IGF-I-GMs재7천내대IGF-I유량호적완만석방작용;BMSCs형태화활성량호;IGF-I-GMs여BMSCs유량호적생물상용성,차촉진BMSCs증치급성골작용균명현우우단독사용IGF-I.
Objective The preparation of the experimental insulin-like growth factor-I gelatin microspheres, the observation of a general nature and in vitro release studies of bone marrow stem cell proliferation and differentiation effects. Method Emulsification cross-linking method prepare IGF-I-GMs and observe its nature;ELISA kit was used to determine the content of IGF-I and calculate the microsphere encapsulation efficiency, drug loading and release pharmaceutical properties in vitro. Bone marrow stem cells were isolated and cultured in vitro application of the MTT assay IGF-I and IGF-I-GMs value-added role of bone marrow stromal cells. Alkaline phosphatase activity the PNPP detection reaction IGF-I and IGF-I-GMs promote the differentiation of bone marrow stromal cells into osteoblasts directed. Result ①The smooth surface of the microspheres prepared, evenly distributed and microsphere drug loading and high encapsulation efficiency of microspheres in vitro drug release good results.②Both IGF-I and IGF-I-GMs group can promote BMSCs value-added, and IGF-I-GMs group was more significant;③Both IGF-I and IGF-I-GMs can promote BMSCs forming osteoblasts, compared to IGF-I, IGF-I-GMs group of the more significant results. Conclusion IGF-I-GMs in 7 days can slow the release of IGF-I meet experimental requirements;IGF-I-GMs good biocompatibility with BMSCs and promote BMSCs appreciation and osteogenic better than the use of IGF-I alone.