CAJ | 학술논문

目的探究大肠癌动物模型的建立及中药(加味痛泻要方)的干预作用.方法将100只小鼠作为研究对象,分为四组(加味痛泻要方剂量高中低三组+对照组),初期给药(DMH)诱发小鼠大肠癌,后采用免疫组化法对四组小鼠突变型P53蛋白表达进行观察,同时给予中药进行后期干预并做好观察记录.结果加味痛泻要方组发癌率明显低于对照组,并且中药干预能明显降低突变型P53蛋白表达.结论中药干预作用明显,对防治大肠癌疗效突出.
목적탐구대장암동물모형적건립급중약(가미통사요방)적간예작용.방법장100지소서작위연구대상,분위사조(가미통사요방제량고중저삼조+대조조),초기급약(DMH)유발소서대장암,후채용면역조화법대사조소서돌변형P53단백표체진행관찰,동시급여중약진행후기간예병주호관찰기록.결과가미통사요방조발암솔명현저우대조조,병차중약간예능명현강저돌변형P53단백표체.결론중약간예작용명현,대방치대장암료효돌출.
Objective To explore the establishment of animal model for colorectal cancer and the intervention effect of traditional chinese medicine (favored pain diarrhea Yaofang). Methods 100 mice as the research object, divided into four groups (favored pain diarrhea Yaofang dose professinally three groups and control group), initial dose (DMH) bath after colorectal cancer in mice induced by immunohistochemical method, four mice mutant P53 protein expression observation, at the same time give chinese medicine for later intervention and make observation record. Results Flavored pain diarrhea Yaofang group hair cancer rate was signifcantly lower than the control group, and traditional chinese medicine intervention can signifcantly reduce the mutant P53 protein expression. Conclusion Chinese medicine intervention has an obvious effect in the prevention of colorectal cancer, curative effect prominent.