中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2013年
11期
625-628
,共4页
汪建超%张晖%朱金海%彭德峰%朱正志%马小开%姚廷敬
汪建超%張暉%硃金海%彭德峰%硃正誌%馬小開%姚廷敬
왕건초%장휘%주금해%팽덕봉%주정지%마소개%요정경
食管鳞癌%miR126%血管内皮生长因子%microRNA
食管鱗癌%miR126%血管內皮生長因子%microRNA
식관린암%miR126%혈관내피생장인자%microRNA
esophageal squamous cell carcinoma%miR126%vascular endothelial growth factor%microRNA
目的:探讨miR126与血管内皮生长因子在食管鳞癌中的表达及关系,以及其对食管鳞癌细胞系的影响.方法:随机选取2011年3月至2011年4月间手术治疗的食管鳞癌患者手术标本4例,,取癌组织及癌旁正常黏膜.提取肿瘤及正常癌旁组织microRNA,逆转录后用实时定量PCR的方法检测miR126的表达情况;提取标本中的mRNA和蛋白用实时定量PCR和Western blot的方法检测VEGF的表达;培养TE-1细胞系并转染miR126模拟体,检测miR126对TE-1细胞增殖和VEGF表达的影响.结果:与正常组织相比,miR126在癌组织的表达明显降低;在RNA和蛋白水平,VEGF水平均明显升高;转染miR126模拟体的细胞BrdU掺入量较转染阴性对照的细胞明显增多,VEGF在RNA和蛋白水平上均明显降低.结论:在食管鳞癌组织中miR126水平下调,VEGF表达增高;miR126可抑制TE-1细胞的增殖,其抑制细胞增殖的作用可能是通过下调VEGF的表达而实现的.
目的:探討miR126與血管內皮生長因子在食管鱗癌中的錶達及關繫,以及其對食管鱗癌細胞繫的影響.方法:隨機選取2011年3月至2011年4月間手術治療的食管鱗癌患者手術標本4例,,取癌組織及癌徬正常黏膜.提取腫瘤及正常癌徬組織microRNA,逆轉錄後用實時定量PCR的方法檢測miR126的錶達情況;提取標本中的mRNA和蛋白用實時定量PCR和Western blot的方法檢測VEGF的錶達;培養TE-1細胞繫併轉染miR126模擬體,檢測miR126對TE-1細胞增殖和VEGF錶達的影響.結果:與正常組織相比,miR126在癌組織的錶達明顯降低;在RNA和蛋白水平,VEGF水平均明顯升高;轉染miR126模擬體的細胞BrdU摻入量較轉染陰性對照的細胞明顯增多,VEGF在RNA和蛋白水平上均明顯降低.結論:在食管鱗癌組織中miR126水平下調,VEGF錶達增高;miR126可抑製TE-1細胞的增殖,其抑製細胞增殖的作用可能是通過下調VEGF的錶達而實現的.
목적:탐토miR126여혈관내피생장인자재식관린암중적표체급관계,이급기대식관린암세포계적영향.방법:수궤선취2011년3월지2011년4월간수술치료적식관린암환자수술표본4례,,취암조직급암방정상점막.제취종류급정상암방조직microRNA,역전록후용실시정량PCR적방법검측miR126적표체정황;제취표본중적mRNA화단백용실시정량PCR화Western blot적방법검측VEGF적표체;배양TE-1세포계병전염miR126모의체,검측miR126대TE-1세포증식화VEGF표체적영향.결과:여정상조직상비,miR126재암조직적표체명현강저;재RNA화단백수평,VEGF수평균명현승고;전염miR126모의체적세포BrdU참입량교전염음성대조적세포명현증다,VEGF재RNA화단백수평상균명현강저.결론:재식관린암조직중miR126수평하조,VEGF표체증고;miR126가억제TE-1세포적증식,기억제세포증식적작용가능시통과하조VEGF적표체이실현적.
Objective:This study aimed to explore miR126 expression and its relationship with vascular endothelial growth factor (VEGF) in esophageal squamous cell carcinoma, as well as the effect of miR126 expression on an esophageal carcinoma cell line. Methods: Four patients with esophageal squamous cell carcinoma were randomly selected in our hospital from 2011.3 to 2011.4. Tumor and normal tumor-adjacent tissue specimens were collected from the patients, and miRNA was extracted. The expression of miR126 was detected by real-time quantitative PCR. Furthermore, total mRNA and protein were extracted to detect VEGF expression by real-time quantitative PCR and Western blot analysis. miR126-like mRNA was then transfected to the TE-1 cell line to determine its effect on the proliferation of TE-1 cells and VEGF expression. Results: miR126 expression was significantly downregulated in esophageal carcinoma tissues compared with normal esophageal tissues. By contrast, the expression of VEGF RNA and protein significantly increased in esophageal carcinoma tissues compared with normal esophageal tissues. The incorporation of BrdU in cells transfected with miR126-like mRNA significantly increased compared with untransfected cells, whereas the levels of VEGF RNA and protein significantly decreased in transfected cells. Conclusion:miR126 expression was downregulated and VEGF was upregulated in esophageal squamous cell carcinoma. Therefore, miR126 may downregulate the expression of VEGF and inhibit the proliferation of TE-1 cells.
