CAJ | 학술논문

目的:探讨溶血磷脂酸(lysophosphatidic acid,LPA)及其受体和IL-6与IL-8在乳腺癌进展中的表达及临床意义.方法:采用半定量RT-PCR方法检测乳腺肿瘤组织和瘤旁组织中LPA受体的表达水平.采用LPA生化测定法和酶联免疫吸附(ELISA)法分别检测乳腺肿瘤患者和健康妇女的血浆LPA、IL-6和IL-8水平.结果:术后复发转移乳腺癌患者血浆LPA、IL-6、IL-8的表达水平均显著高于局限期乳腺癌和良性乳腺肿瘤患者及健康妇女(P均<0.05);LPA1在乳腺癌组织中的表达水平显著高于良性乳腺肿瘤组织和正常乳腺组织(P<0.05);乳腺癌患者血浆LPA水平与IL-6和IL-8水平均呈正相关(P均<0.01).结论:LPA对乳腺癌患者内源性IL-6和IL-8的表达可能具有上调作用,检测LPA、IL-6和IL-8的表达水平对乳腺癌的转移可能有一定的预测作用,尤其是骨转移.
목적:탐토용혈린지산(lysophosphatidic acid,LPA)급기수체화IL-6여IL-8재유선암진전중적표체급림상의의.방법:채용반정량RT-PCR방법검측유선종류조직화류방조직중LPA수체적표체수평.채용LPA생화측정법화매련면역흡부(ELISA)법분별검측유선종류환자화건강부녀적혈장LPA、IL-6화IL-8수평.결과:술후복발전이유선암환자혈장LPA、IL-6、IL-8적표체수평균현저고우국한기유선암화량성유선종류환자급건강부녀(P균<0.05);LPA1재유선암조직중적표체수평현저고우량성유선종류조직화정상유선조직(P<0.05);유선암환자혈장LPA수평여IL-6화IL-8수평균정정상관(P균<0.01).결론:LPA대유선암환자내원성IL-6화IL-8적표체가능구유상조작용,검측LPA、IL-6화IL-8적표체수평대유선암적전이가능유일정적예측작용,우기시골전이.
Objective:This work aimed to investigate the expression level of lysophosphatidic acid (LPA) and its receptors. The paper also discussed the interrelationship among the LPA, the receptors, and IL-6 and IL-8 in breast cancer tissues. Methods:The ex-pressions of the 3 hypo-types of LPA receptor in the breast cancer and paraneoplastic tissues were detected using semi-quantitative re-verse transcription polymerase chain reaction. The plasma levels of LPA, IL-6 and IL-8 were respectively detected in healthy subjects and in patients with benign breast tumor using the LPA biochemistry and enzyme linked immunosorbent assay kits. Results:The plas-ma LPA level was significantly higher in patients with breast cancer metastasis than in those with local breast cancer (P<0.01), benign breast tumor (P<0.01), and healthy volunteers (P<0.01). In addition, the IL-6 and IL-8 plasma levels were higher in the group with me-tastasis compared with the other three groups, too (P<0.01). LPA1 expression level was higher in breast cancer tissue than in benign breast tumor (P<0.05) and in normal breast tissue (P<0.05). There was a significantly positive correlation between the plasma LPA and the plasma IL-6 in patients with breast cancer (P<0.01), and between the plasma LPA and IL-8 (P<0.01). Conclusion:LPA expressions on the endogenous IL-6 and IL-8 in patients with breast cancer may have an up-regulation. Moreover, the detection of the LPA, IL-6, and IL-8 expression levels may have some predictable effects on metastatic breast cancer, especially bone metastases.