浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2013年
6期
440-442
,共3页
大鼠%缺血再灌注%基质金属蛋白酶-9%血脑屏障%葛根素
大鼠%缺血再灌註%基質金屬蛋白酶-9%血腦屏障%葛根素
대서%결혈재관주%기질금속단백매-9%혈뇌병장%갈근소
rats%ischemia-reperfusion%matrix metalloproteinase-9%blood-brain%barriar puerarin
目的:观察葛根素对大鼠局灶性脑缺血再灌注后脑组织基质金属蛋白酶-9(MMP-9)表达和脑组织含水率变化的影响,探讨葛根素脑保护的可能机制.方法:54只雄性SD大鼠分为假手术组、对照组和药物组,每组18只.采用线栓法建立右侧大脑中动脉闭塞大鼠模型,时间点为再灌注48h,采用比色法测定脑组织中伊文思蓝(EB)含量反映血脑屏障通透性;干-湿重法检测脑组织含水率;免疫组化法检测脑组织MMP-9表达.结果:脑缺血再灌注后大鼠脑缺血区MMP-9表达明显增加(P<0.05),脑组织含水率明显升高(P<0.05).与对照组比较,药物组大鼠脑组织MMP-9明显低表达(P<0.05),脑组织含水率明显减少(P<0.05).结论:葛根素可能通过抑制MMP-9表达来减轻血脑屏障破坏和脑水肿而发挥缺血再灌注损伤时的脑保护作用.
目的:觀察葛根素對大鼠跼竈性腦缺血再灌註後腦組織基質金屬蛋白酶-9(MMP-9)錶達和腦組織含水率變化的影響,探討葛根素腦保護的可能機製.方法:54隻雄性SD大鼠分為假手術組、對照組和藥物組,每組18隻.採用線栓法建立右側大腦中動脈閉塞大鼠模型,時間點為再灌註48h,採用比色法測定腦組織中伊文思藍(EB)含量反映血腦屏障通透性;榦-濕重法檢測腦組織含水率;免疫組化法檢測腦組織MMP-9錶達.結果:腦缺血再灌註後大鼠腦缺血區MMP-9錶達明顯增加(P<0.05),腦組織含水率明顯升高(P<0.05).與對照組比較,藥物組大鼠腦組織MMP-9明顯低錶達(P<0.05),腦組織含水率明顯減少(P<0.05).結論:葛根素可能通過抑製MMP-9錶達來減輕血腦屏障破壞和腦水腫而髮揮缺血再灌註損傷時的腦保護作用.
목적:관찰갈근소대대서국조성뇌결혈재관주후뇌조직기질금속단백매-9(MMP-9)표체화뇌조직함수솔변화적영향,탐토갈근소뇌보호적가능궤제.방법:54지웅성SD대서분위가수술조、대조조화약물조,매조18지.채용선전법건립우측대뇌중동맥폐새대서모형,시간점위재관주48h,채용비색법측정뇌조직중이문사람(EB)함량반영혈뇌병장통투성;간-습중법검측뇌조직함수솔;면역조화법검측뇌조직MMP-9표체.결과:뇌결혈재관주후대서뇌결혈구MMP-9표체명현증가(P<0.05),뇌조직함수솔명현승고(P<0.05).여대조조비교,약물조대서뇌조직MMP-9명현저표체(P<0.05),뇌조직함수솔명현감소(P<0.05).결론:갈근소가능통과억제MMP-9표체래감경혈뇌병장파배화뇌수종이발휘결혈재관주손상시적뇌보호작용.
Objective: To investigate the influence of puerarin on activation of matrix metalloproteinase-9 (MMP-9) in brain tissue and brain edema after cerebral ischemia-reperfusion in rats. Methods: A total 54 rats were randomly assigned to sham-operated(sham) group,control group,and medicine group.Puerarin was given at dose of 70 mg/kg to the medicine group 1 h before ischemia and every 8 h afterwards,and the equivalent amount of normal saline was given to the sham and control groups. The middle cerebral artery of the rats was occluded and recirculated at 48 h. At this time point, the permeability of blood-brain barrier was evaluated based on the leakage of Evans blue. Cerebral edema formation was determined by brain water content. The ex?pression of MMP-9 was measured by immunohistochemistry. Results: Immunohistochemistry revealed an increase of MMP-9 at 48 h post-reperfusion in the control and medicine groups(P<0.05). Puerarin decreased the level of MMP-9 and brain water content in the medicine group as compared to those in the control group(P<0.05). Conclusion: Puerarin may attenuate ischemia-reperfusion injury through inhibition of MMP-9 activation and brain edema.
