CAJ | 학술논문

采用正相高效液相色谱法测定200份不同省份来源的谷子育成品种籽粒中的4种维生素E(α-VE、β-VE、γ-VE和δ-VE)及叶黄素和玉米黄素2种类胡萝卜素组分的含量,并分析了谷子维生素E含量与类胡萝卜素含量和主要农艺性状的相关性.结果显示,我国谷子育成品种籽粒中VE总量、α-VE含量及(β+γ)-VE含量均呈正态分布,δ-VE含量呈偏态分布,且(β+γ)-VE是维生素E的主要组分;陕西和吉林谷子的VE总量和(β+γ)-VE含量显著高于其他省份品种,东北三省谷子的α-VE含量显著高于其他省份,而不同省份谷子品种中δ-VE含量差异不显著;不同省份谷子品种间叶黄素含量差异达极显著(P<0.01),玉米黄素含量差异不显著,类胡萝卜素总含量差异显著(P<0.05).相关分析表明,参试品种籽粒中叶黄素含量与 VE 总量呈显著正相关,与α-VE 含量呈极显著正相关,与δ-VE 含量呈极显著负相关,与(β+γ)-VE含量不相关;玉米黄素与VE总量和α-VE含量呈显著正相关,与δ-VE含量呈极显著负相关,与(β+γ)-VE含量不著相关;VE总量和(β+γ)-VE含量与千粒重呈显著负相关,δ-VE含量与粒重和穗重呈极显著正相关,类胡萝卜素各组分与草重呈极显著正相关.
채용정상고효액상색보법측정200빈불동성빈래원적곡자육성품충자립중적4충유생소E(α-VE、β-VE、γ-VE화δ-VE)급협황소화옥미황소2충류호라복소조분적함량,병분석료곡자유생소E함량여류호라복소함량화주요농예성상적상관성.결과현시,아국곡자육성품충자립중VE총량、α-VE함량급(β+γ)-VE함량균정정태분포,δ-VE함량정편태분포,차(β+γ)-VE시유생소E적주요조분;협서화길림곡자적VE총량화(β+γ)-VE함량현저고우기타성빈품충,동북삼성곡자적α-VE함량현저고우기타성빈,이불동성빈곡자품충중δ-VE함량차이불현저;불동성빈곡자품충간협황소함량차이체겁현저(P<0.01),옥미황소함량차이불현저,류호라복소총함량차이현저(P<0.05).상관분석표명,삼시품충자립중협황소함량여 VE 총량정현저정상관,여α-VE 함량정겁현저정상관,여δ-VE 함량정겁현저부상관,여(β+γ)-VE함량불상관;옥미황소여VE총량화α-VE함량정현저정상관,여δ-VE함량정겁현저부상관,여(β+γ)-VE함량불저상관;VE총량화(β+γ)-VE함량여천립중정현저부상관,δ-VE함량여립중화수중정겁현저정상관,류호라복소각조분여초중정겁현저정상관.
We determined vitamin E (VE) and carotenoid contents in 200 foxtail millet (Setaria italica Beauv.) developed varieties from different provinces with high performance liquid chromatography (HPLC) to explore distribution and screen varieties with high VE and carotenoid contents, as well as analyze correlations among total VE content, α-VE content, (β+γ)-VE content, δ-VE content, carotenoid content and agronomic traits. The results showed that the distribution of total VE content,α-VE content and (β+γ)-VE content in foxtail millet varieties were normal, while that of δ-VE content was skwe. (β+γ)-VE content was the most abundant fraction in china foxtail millet varieties. The foxtail millet varieties from Shaanxi and Jilin had the highest total VE con-tents with significant difference from those of other provinces;those from Heilongjiang, Jilin and Liaoning had highest (β+γ)-VE contents also with significant difference from those of other provinces. There was no significant difference inδ-VE content among varieties of different provinces. Lutein and zeaxanthin were two main carotenoids in foxtail millet varieties. The contents of these carotenoids were extremely different among foxtail millet varieties which came from different provinces. Correlation analysis showed that lutein and zeaxanthin contents were significantly and positively correlated with the total VE content andα-VE content, while significantly and negatively correlated with theδ-VE content. There was no significant correlation between lutein content and (β+γ)-VE content. Total VE content and (β+γ)-VE content were all negatively correlated with 1000-seed weight at 0.05 prob-ability level,δ-VE content was positively correlated with weed weight per plant and spikelet weight at 0.01 probability level. Ca-rotenoid contents were all positively correlated with grass weight at 0.01 probability level.