作物学报
作物學報
작물학보
ACTA AGRONOMICA SINICA
2013年
3期
423-430
,共8页
蔺芳芳%杨旭%武小翠%刘晓梅%葛荣朝%赵宝存*
藺芳芳%楊旭%武小翠%劉曉梅%葛榮朝%趙寶存*
린방방%양욱%무소취%류효매%갈영조%조보존*
TaSC%小麦%cDNA表达文库%分裂泛素酵母双杂交%双分子荧光互补技术
TaSC%小麥%cDNA錶達文庫%分裂汎素酵母雙雜交%雙分子熒光互補技術
TaSC%소맥%cDNA표체문고%분렬범소효모쌍잡교%쌍분자형광호보기술
TaSC%Triticum asetivum L.%cDNA expression library%Split ubiquitin yeast two-hybrid system%BiFC
TaSC (Triticum asetivum L. salt-tolerance related gene, GenBank 登录号为 AY956330)是从小麦耐盐突变体RH8706-49中克隆的高盐诱导表达的耐盐相关基因.以该基因编码的蛋白质TaSC为诱饵,运用分裂泛素酵母双杂交技术从小麦 cDNA 表达文库中钓取互作蛋白质,筛选到一个编码小麦未知功能蛋白质的基因(GenBank 登录号为AK336035),命名为TaSCIP1(TaSC interaction protein 1).双分子荧光互补(BiFC)实验证实TaSCIP1与TaSC存在互作.该互作蛋白的分离有利于进一步研究TaSC基因的耐盐机制.
TaSC (Triticum asetivum L. salt-tolerance related gene, GenBank 登錄號為 AY956330)是從小麥耐鹽突變體RH8706-49中剋隆的高鹽誘導錶達的耐鹽相關基因.以該基因編碼的蛋白質TaSC為誘餌,運用分裂汎素酵母雙雜交技術從小麥 cDNA 錶達文庫中釣取互作蛋白質,篩選到一箇編碼小麥未知功能蛋白質的基因(GenBank 登錄號為AK336035),命名為TaSCIP1(TaSC interaction protein 1).雙分子熒光互補(BiFC)實驗證實TaSCIP1與TaSC存在互作.該互作蛋白的分離有利于進一步研究TaSC基因的耐鹽機製.
TaSC (Triticum asetivum L. salt-tolerance related gene, GenBank 등록호위 AY956330)시종소맥내염돌변체RH8706-49중극륭적고염유도표체적내염상관기인.이해기인편마적단백질TaSC위유이,운용분렬범소효모쌍잡교기술종소맥 cDNA 표체문고중조취호작단백질,사선도일개편마소맥미지공능단백질적기인(GenBank 등록호위AK336035),명명위TaSCIP1(TaSC interaction protein 1).쌍분자형광호보(BiFC)실험증실TaSCIP1여TaSC존재호작.해호작단백적분리유리우진일보연구TaSC기인적내염궤제.
The TaSC (Triticum asetivum L. salt-tolerance related gene, GenBank accession number AY956330) has been cloned from wheat salt tolerant mutant RH 8706-49, which is induced by high salinity. To fish interacting proteins of this gene, we used TaSC protein as bait to hybrid with the wheat cDNA expression library using the split ubiquitin yeast two hybrid system in this experiment. A novel wheat gene (GenBank accession number AK336035) encoding a protein with unknown function was isolated, which was designated TaSCIP1 (TaSC interaction protein 1). Bimolecular fluorescence complementation (BiFC) experiment con-firmed the interaction between proteins TaSCIP1 and TaSC. The separation of TaSC-interacting protein is beneficial to disclose the mechanism of salt tolerance gene TaSC.