CAJ | 학술논문

为进一步明确玉米株高和穗位高的遗传机制,为育种生产提供服务,本研究以K22×CI7、K22×Dan340的F2群体为作图群体,利用覆盖玉米10条染色体的SNP标记构建了2个连锁图谱.并将这2个F2群体衍生的分别含237和218个家系的F2:3群体用于田间性状的鉴定.用复合区间作图模型对2个群体的株高、穗位高表型进行QTL定位分析.结果显示,在广西南宁和湖北武汉两种环境条件下共定位到21个株高 QTL 和27个穗位高 QTL;单个 QTL表型变异贡献率的变幅为4.9%~17.9%;株高和穗位高QTL的作用方式以加性和部分显性为主;第7染色体上可能存在控制株高和穗位高的主效QTL.
위진일보명학옥미주고화수위고적유전궤제,위육충생산제공복무,본연구이K22×CI7、K22×Dan340적F2군체위작도군체,이용복개옥미10조염색체적SNP표기구건료2개련쇄도보.병장저2개F2군체연생적분별함237화218개가계적F2:3군체용우전간성상적감정.용복합구간작도모형대2개군체적주고、수위고표형진행QTL정위분석.결과현시,재엄서남저화호북무한량충배경조건하공정위도21개주고 QTL 화27개수위고 QTL;단개 QTL표형변이공헌솔적변폭위4.9%~17.9%;주고화수위고QTL적작용방식이가성화부분현성위주;제7염색체상가능존재공제주고화수위고적주효QTL.
In order to learn more about the genetic machanism of plant height and ear height, two linkage maps were constructed by SNP markers using two F2:3 families derived from K22 × CI7 and K22 × Dan340, respectively. These two maps included 429 and 344 polymorphic SNP markers respectively and their total lengths were 1 389.3 cM and 1 567.5 cM respectively. The pheno-typic data of plant height (PH) and ear height (EH) of two populations were used to detect QTLs in two environments (2010 in Nanning of Guangxi Province and 2011 in Wuhan of Hubei Province) by using the Composite Interval Mapping (CIM) model of WinQTLCart2.5. In total, 21 QTLs for plant height and 27 QTLs for ear height were identified. The phenotypic variance ex-plained by each QTL ranged from 4.9%to 17.9%. The results showed that the additive and partial dominant effects were the main genetic basis for plant height and ear height in maize in this study, and the main QTLs for PH and EH were both found on chro-mosome 7.