CAJ | 학술논문

富含脯氨酸的蛋白(proline-rich proteins, PRPs)代表一类富含脯氨酸和羟脯氨酸的细胞壁结构蛋白质,最先在伤害诱导的胡萝卜贮藏根中被发现.越来越多的证据显示这类蛋白在应答多种生物和非生物胁迫中起作用.我们之前从棉花cDNA文库中分离了一个命名为GhPRP5的编码富含脯氨酸蛋白的基因,为研究其功能,构建了GhPRP5的过量表达载体,转化拟南芥,获得GhPRP5高表达的8个株系的纯合体.在正常培养条件下,转基因株系和野生型种子的萌发率一致,但盐胁迫和ABA处理显著抑制了转基因拟南芥株系种子的萌发.盐胁迫条件下野生型的绿苗率明显高于转基因拟南芥株系,与正常生长条件相比, ABA 处理抑制转基因拟南芥主根伸长的程度更大.利用Quantitative RT-PCR技术分析几个胁迫相关标记基因的表达情况表明,盐和ABA诱导了RD29A、RD29B和KIN1的表达,但诱导水平在转基因株系和野生型中不一样,说明 GhPRP5参与调控拟南芥胁迫相关标记基因的表达,但具体参与的胁迫应答信号传导途径仍需进一步研究.
부함포안산적단백(proline-rich proteins, PRPs)대표일류부함포안산화간포안산적세포벽결구단백질,최선재상해유도적호라복저장근중피발현.월래월다적증거현시저류단백재응답다충생물화비생물협박중기작용.아문지전종면화cDNA문고중분리료일개명명위GhPRP5적편마부함포안산단백적기인,위연구기공능,구건료GhPRP5적과량표체재체,전화의남개,획득GhPRP5고표체적8개주계적순합체.재정상배양조건하,전기인주계화야생형충자적맹발솔일치,단염협박화ABA처리현저억제료전기인의남개주계충자적맹발.염협박조건하야생형적록묘솔명현고우전기인의남개주계,여정상생장조건상비, ABA 처리억제전기인의남개주근신장적정도경대.이용Quantitative RT-PCR기술분석궤개협박상관표기기인적표체정황표명,염화ABA유도료RD29A、RD29B화KIN1적표체,단유도수평재전기인주계화야생형중불일양,설명 GhPRP5삼여조공의남개협박상관표기기인적표체,단구체삼여적협박응답신호전도도경잉수진일보연구.
Pro-rich proteins (PRPs) represent one family of Pro-and Hyp-rich structural cell wall proteins that are initially identi-fied as wound-induced gene products in carrot storage roots. Accumulated evidences demonstrate that PRP genes are regulated by various abiotic and biotic stresses and may play a role in plant responses to changes in living conditions. In our previous study, a gene encoding a proline-rich protein designated as GhPRP5 was isolated from cotton cDNA libraries. To validate its function, in this study, we introduced the coding region of GhPRP5 into the vector pBI121 under the control of the CaMV 35S promoter and then transformed the vector into Arabidopsis thaliana. Eight independent T4 homozygous lines with high expression of GhPRP5 were obtained. Germination rate of transgenic lines overexpressing GhPRP5 was not affected under normal conditions;however, salt stress and ABA significantly inhibited the germination of the transgenic lines. When growing on media with NaCl, the GhPRP5-overexpressed plants displayed much less cotyledon greening rate compared with the wild type. In contrast to the normal growth conditions, ABA inhibited the elongation of primary root more severely in the transgenic lines. Quantitative RT-PCR tech-nique was used to analyze the transcription of several stress gene markers (RD29A, RD29B, KIN1, and ABI1) in the transgenic lines and the wild type plants under salt stress and ABA treatments. Expressions of RD29A, RD29B, and KIN1 were induced by ABA and NaCl in the transgenic and the wild type plants, though the induction levels in the transgenic lines were different from those in the wild type. This finding suggests that GhPRP5 is implicated in the regulation of stress gene expression in Arabidopsis. The plant stress signal transduction pathway in which GhPRP5 may be involved needs to be further studied.