CAJ | 학술논문

以耐寒性较强的三门峡栝楼和耐寒性较弱的安庆栝楼为实验材料,研究了低温(4℃)胁迫下喷施不同浓度(0,0.25,0.5,1和2 mmol/L)的水杨酸溶液对栝楼幼苗叶片生理生长特性及抗氧化酶活性的影响.实验结果表明：在低温处理下,2个品种栝楼幼苗的细胞膜透性、游离脯氨酸( Pro)含量和可溶性蛋白含量均增加(P<0.05),而相对水含量出现了相反的变化趋势,植株超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶( PPO)、酯酶( EST)和细胞色素氧化酶( CYT)的活性呈现增加的现象,显示出2个栝楼品种的耐寒性差异；喷施低浓度的水杨酸溶液能够显著降低植株的相对电导率、质膜透性及Pro和可溶性蛋白的含量,而高浓度的水杨酸对于降低植株SOD,POD,PPO,EST和CYT的活性均没有低浓度的显著.由此可知,适宜浓度的水杨酸有缓解栝楼低温胁迫的效果,但不能消除冷胁迫对栝楼生长的抑制作用.外源水杨酸缓解栝楼低温胁迫的最适浓度是0.5 mmol/L.
이내한성교강적삼문협괄루화내한성교약적안경괄루위실험재료,연구료저온(4℃)협박하분시불동농도(0,0.25,0.5,1화2 mmol/L)적수양산용액대괄루유묘협편생리생장특성급항양화매활성적영향.실험결과표명：재저온처리하,2개품충괄루유묘적세포막투성、유리포안산( Pro)함량화가용성단백함량균증가(P<0.05),이상대수함량출현료상반적변화추세,식주초양화물기화매(SOD)、과양화물매(POD)、다분양화매( PPO)、지매( EST)화세포색소양화매( CYT)적활성정현증가적현상,현시출2개괄루품충적내한성차이；분시저농도적수양산용액능구현저강저식주적상대전도솔、질막투성급Pro화가용성단백적함량,이고농도적수양산대우강저식주SOD,POD,PPO,EST화CYT적활성균몰유저농도적현저.유차가지,괄의농도적수양산유완해괄루저온협박적효과,단불능소제랭협박대괄루생장적억제작용.외원수양산완해괄루저온협박적최괄농도시0.5 mmol/L.
Two kinds of Trichosanthes kirilowii Maxim, one was sanmenxia Trichosanthes kirilowii with strong cold resistance and the other one was Anqing Trichosanthes kirilowii with weak cold resistance, were taken as the experiment materials, to analyze the effect of physical growth characteristics and autioxidant enzyme activi-ties in spraying salicylic acid with different concentrations (0, 0. 25, 0. 5, 1, 2 mmol/L) for Trichosanthes kirilowii seedling leaves under the low temperature ( 4 ℃) stress. The experimental results showed that for Trichosanthes kirilowii seedling treatment under low temperature, the cell membrane permeability and free pro-line (Pro) and soluble protein contents of two kinds of Trichosanthes kirilowii increase (P<0. 05), while the relative water content ( RWC) appeared the opposite tendency, and the plant superoxide dismutase ( SOD) , peroxidase ( POD) , polyphenol oxidase ( PPO) , esterase ( EST) and cytochrome oxidase ( CYT) increased, showing the cold resistance difference between two kinds of Trichosanthes kirilowii, Spaying SA in appropriate low concentration significantly reduced the relative conductivity, plasma membrane permeability and Pro and soluble protein contents, but spaying SA in high concentration did not affect reducing the contents of SOD, POD, PPO, EST and CYT of plants. The SA in appropriate concentration had the effect of alleviating the Tri-chosanthes kirilowii under the low temperature stress, but did not eliminate the inhibition of cold stress on Tri-chosanthes kirilowii growth. The optimal concentration of exogenous salicylic acid to alleviate Trichosanthes kirilowii was 0. 5 mmol/L.