浙江师范大学学报（自然科学版）
浙江師範大學學報（自然科學版）
절강사범대학학보（자연과학판）
JOURNAL OF ZHEJIANG NORMAL UNIVERSITY(NATURAL SCIENCES)
2015年
1期
1-8
,共8页
慢性氟中毒%海马%突触后致密蛋白-95%学习记忆%膜流动性
慢性氟中毒%海馬%突觸後緻密蛋白-95%學習記憶%膜流動性
만성불중독%해마%돌촉후치밀단백-95%학습기억%막류동성
chronic fluorosis%hippocampus%PSD-95%learning and memory ability%membrane fluidity
探讨了慢性氟中毒致学习记忆损伤的脑内机制。选用初断乳雄性SD大鼠192只,随机分为4组：1个对照组,饮用自来水；3个分别饮用15,30和60 mg/L NaF溶液的染氟组。染氟期为18月。每3月用开场行为和Morris水迷宫法检测大鼠的学习记忆行为；分别在染氟中期(9月)和染氟结束后(18月)分2批断头处死大鼠,进行脑海马突触体膜流动性和海马CA3区突触后致密蛋白-95( PSD-95)表达水平等检测。结果表明：慢性氟中毒致大鼠自发活动和探究行为显著或极显著抑制,空间学习记忆能力显著下降；脑海马突触膜流动性、PSD-95表达水平均显著下降。提示慢性氟中毒致脑海马突触体膜流动性和突触后致密蛋白-95表达水平的改变可能是慢性氟中毒致学习记忆损伤的脑内突触机制之一。
探討瞭慢性氟中毒緻學習記憶損傷的腦內機製。選用初斷乳雄性SD大鼠192隻,隨機分為4組：1箇對照組,飲用自來水；3箇分彆飲用15,30和60 mg/L NaF溶液的染氟組。染氟期為18月。每3月用開場行為和Morris水迷宮法檢測大鼠的學習記憶行為；分彆在染氟中期(9月)和染氟結束後(18月)分2批斷頭處死大鼠,進行腦海馬突觸體膜流動性和海馬CA3區突觸後緻密蛋白-95( PSD-95)錶達水平等檢測。結果錶明：慢性氟中毒緻大鼠自髮活動和探究行為顯著或極顯著抑製,空間學習記憶能力顯著下降；腦海馬突觸膜流動性、PSD-95錶達水平均顯著下降。提示慢性氟中毒緻腦海馬突觸體膜流動性和突觸後緻密蛋白-95錶達水平的改變可能是慢性氟中毒緻學習記憶損傷的腦內突觸機製之一。
탐토료만성불중독치학습기억손상적뇌내궤제。선용초단유웅성SD대서192지,수궤분위4조：1개대조조,음용자래수；3개분별음용15,30화60 mg/L NaF용액적염불조。염불기위18월。매3월용개장행위화Morris수미궁법검측대서적학습기억행위；분별재염불중기(9월)화염불결속후(18월)분2비단두처사대서,진행뇌해마돌촉체막류동성화해마CA3구돌촉후치밀단백-95( PSD-95)표체수평등검측。결과표명：만성불중독치대서자발활동화탐구행위현저혹겁현저억제,공간학습기억능력현저하강；뇌해마돌촉막류동성、PSD-95표체수평균현저하강。제시만성불중독치뇌해마돌촉체막류동성화돌촉후치밀단백-95표체수평적개변가능시만성불중독치학습기억손상적뇌내돌촉궤제지일。
The synaptic mechanism of learning-memory injury induced by chronic fluorosis in brain was inves-tigated. 192 Sprague-Dawley ( SD) male rats, one-month-old, were randomly divided into four groups and given 15, 30, 60 mg/L NaF solution and tap water respectively for 18 months. Behavior of rats was evaluated by open field and Morris Water Maze test every 3 months. The hippocampal synaptic membrane fluidity was detected by electron spin resonance, the expression level of post synaptic density 95 (PSD-95) in hippocam-pal CA3 region was detected by immunohistochemistry. Results showed that in rats with chronic fluorosis com-pared with the controls, locomotor activity and exploratory behavior were significantly or very significantly sup-pressed, spatial learning and memory ability were significantly declined; synaptic membrane fluidity and the protein level of PSD-95 of hippocampus were greatly decreased. The data indicated that the changes of synap-tosome membrane fluidity and PSD-95 expression level in hippocampus might be the one synaptic mechanism of learning-memory injury induced by chronic fluorosis in brain.
