国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2014年
21期
1606-1609
,共4页
谭焰%季磊%谷伟%孙丽华
譚燄%季磊%穀偉%孫麗華
담염%계뢰%곡위%손려화
慢性阻塞性肺疾病%噻托溴铵%膈肌细胞%凋亡
慢性阻塞性肺疾病%噻託溴銨%膈肌細胞%凋亡
만성조새성폐질병%새탁추안%격기세포%조망
Chronic obstructive pulmonary disease%Tiotropium bromide%Diaphragmatic muscle cell%Apoptosis
目的 探讨噻托溴铵对COPD大鼠膈肌细胞过度凋亡的调节作用及其机制.方法 60只雄性Wistar大鼠随机分成健康对照组、COPD组和噻托溴铵干预组.采用烟熏及气管内注射脂多糖建立COPD模型,第91天将大鼠处死,观察各组大鼠肺组织的病理变化,TUNEL法检测各组膈肌细胞的凋亡率,免疫组织化学法检测膈肌细胞内Fas蛋白的表达,Westren blot法检测膈肌细胞中FasL及Caspase 3的含量,RT-PCR法检测Caspase 3基因表达.结果 噻托溴铵组大鼠膈肌细胞的凋亡率、Fas、FasL、Caspase-3蛋白含量及Caspase-3基因表达均低于COPD组,高于健康对照组,3组之间差异有统计学意义(P<0.05).结论 COPD的发生发展过程中存在膈肌细胞过度凋亡,Fas/FasL途径参与了膈肌细胞凋亡的调控,噻托溴铵可抑制膈肌细胞的过度凋亡,延缓COPD的进程.
目的 探討噻託溴銨對COPD大鼠膈肌細胞過度凋亡的調節作用及其機製.方法 60隻雄性Wistar大鼠隨機分成健康對照組、COPD組和噻託溴銨榦預組.採用煙熏及氣管內註射脂多糖建立COPD模型,第91天將大鼠處死,觀察各組大鼠肺組織的病理變化,TUNEL法檢測各組膈肌細胞的凋亡率,免疫組織化學法檢測膈肌細胞內Fas蛋白的錶達,Westren blot法檢測膈肌細胞中FasL及Caspase 3的含量,RT-PCR法檢測Caspase 3基因錶達.結果 噻託溴銨組大鼠膈肌細胞的凋亡率、Fas、FasL、Caspase-3蛋白含量及Caspase-3基因錶達均低于COPD組,高于健康對照組,3組之間差異有統計學意義(P<0.05).結論 COPD的髮生髮展過程中存在膈肌細胞過度凋亡,Fas/FasL途徑參與瞭膈肌細胞凋亡的調控,噻託溴銨可抑製膈肌細胞的過度凋亡,延緩COPD的進程.
목적 탐토새탁추안대COPD대서격기세포과도조망적조절작용급기궤제.방법 60지웅성Wistar대서수궤분성건강대조조、COPD조화새탁추안간예조.채용연훈급기관내주사지다당건립COPD모형,제91천장대서처사,관찰각조대서폐조직적병리변화,TUNEL법검측각조격기세포적조망솔,면역조직화학법검측격기세포내Fas단백적표체,Westren blot법검측격기세포중FasL급Caspase 3적함량,RT-PCR법검측Caspase 3기인표체.결과 새탁추안조대서격기세포적조망솔、Fas、FasL、Caspase-3단백함량급Caspase-3기인표체균저우COPD조,고우건강대조조,3조지간차이유통계학의의(P<0.05).결론 COPD적발생발전과정중존재격기세포과도조망,Fas/FasL도경삼여료격기세포조망적조공,새탁추안가억제격기세포적과도조망,연완COPD적진정.
Objective Explore the influence and mechanism of tiotropium bromide on the excessive apoptosis of diaphragmatic muscle in chronic obstructive pulmonary disease (COPD) rats.Methods Sixty male Wistar rats are randomly divided into control group,COPD group and tiotropium Bromide group.The rat models of COPD were established by passive smoking as well as intratracheal instillation of lipopolysaccharide respectively.The pathologic change of the lung tissue and airway were observed by HE staining.The diaphragmatic muscle cell apoptosis rate,Fas,FasL and Caspase-3 protein and Caspase-3 gene expression of diaphragmatic muscle cell were measured.Results HE staining proved that the rat COPD models were successfully established.The COPD group appears obvious emphysema,while the tiotropium bromide group appears mild emphysema.The apoptosis rate,Fas,FasL and Caspase-3 protein and Caspase-3 gene expression of diaphragmatic muscle cell in tiotropium bromide group were higher than the healthy control group,but less than COPD group (P < 0.05).Conclusions Excessive apoptosis of diaphragmatic muscle cell was present in rats with COPD.Fas/FasL mediated apoptosis way was involved in diaphragm apoptosis.Tiotropium bromide can reduce the level of apoptosis in diaphragmatic muscle cell.