CAJ | 학술논문

目的：建立一种脊髓损伤椎管内蛛网膜下腔持续给药的动物模型。方法参照A llen重物坠落法制备急性脊髓挫伤动物模型，模拟临床上外伤所致的脊髓损伤，并在损伤灶安装蛛网膜下腔持续给药装置，观察大鼠的行为表现，并通过检测给药装置内葡萄糖含量判断该装置效果。结果脊髓损伤实验组大鼠麻醉清醒后均表现为双后肢截瘫，损伤平面以下肌张力降低，肌力0级，对针刺亦无反应，并明显尿潴留；对照组大鼠四肢活动正常。30只大鼠中，1只在术后2 d死亡，2只大鼠表现为痉挛性瘫痪，2只大鼠导管脱落或堵塞，6只大鼠表现出不同程度的自残现象。结论按照A llen重物坠落法制备急性脊髓挫伤并在损伤灶安装蛛网膜下腔持续给药装置的动物模型，重复性较好，获得稳定的伤动物模型。
목적：건립일충척수손상추관내주망막하강지속급약적동물모형。방법삼조A llen중물추락법제비급성척수좌상동물모형，모의림상상외상소치적척수손상，병재손상조안장주망막하강지속급약장치，관찰대서적행위표현，병통과검측급약장치내포도당함량판단해장치효과。결과척수손상실험조대서마취청성후균표현위쌍후지절탄，손상평면이하기장력강저，기력0급，대침자역무반응，병명현뇨저류；대조조대서사지활동정상。30지대서중，1지재술후2 d사망，2지대서표현위경련성탄탄，2지대서도관탈락혹도새，6지대서표현출불동정도적자잔현상。결론안조A llen중물추락법제비급성척수좌상병재손상조안장주망막하강지속급약장치적동물모형，중복성교호，획득은정적상동물모형。
Objective To establish spinal cord injury rat model of continuous infusion inside subarachnoid space of spinal canal .Methods By using Allen′s method as a reference ,rat models of acute spinal canal injury was prepared to simulate spinal cord injuries similar to clinical trauma .Continuous infusion device was installed inside subarachnoid space in the lesion .The behaviors of rat models were observed and the effects of this device was evalua‐ted by measuring the level of glucose in the drug delivery device .Results Rats in the experimental group suffered from paraplegia in double hindlimb ,and the tension of muscle below the level of spinal injury was decreased ,with myodynamia level of 0 grade ,without reaction to acupuncture ,and with apparent urine retention .The four limbs of rats in the control group functioned normally .Among all of the 30 rats ,1 rat died in 2 days ,2 rats suffered from spas‐tic paralysis ,catheter of 2 rats were lost or blocked ,and 6 rats showed various degree of self mutilation after surgery . Conclusion The rat model of acute spinal canal injury ,prepared in reference to Allen′s method and installed with continuous infusion device inside subarachnoid space in the lesion ,might be with fine repeatability and could provide stable injured rat model .