CAJ | 학술논문

目的探讨臭氧对帕金森病(PD)大鼠模型黑质氧化应激及凋亡相关蛋白表达的影响. 方法 SD大鼠32只按随机数字表法分为PD组、20 mg/L臭氧治疗组、50 mg/L臭氧治疗组和正常对照组,每组8只,前3组大鼠右侧纹状体注射15μg6-羟多巴胺(6-OHDA)制备PD模型,正常对照组注射等量生理盐水;20、50 mg/L臭氧治疗组分别在造模后4周经直肠吹入5.0 mL氧气和臭氧的混合气体(臭氧浓度分别为20 mg/L和50 mg/L,剂量为1 mg/kg),连续15d.相应处理后取大鼠中脑黑质脑组织,采用化学比色法测定黑质区脂质过氧化产物丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽超氧化物酶(GSH-Px)的浓度;采用Western blotting检测黑质内凋亡相关蛋白B细胞淋巴瘤基因-2(Bcl-2)和Bcl-2相关X蛋白(Bax)的表达. 结果与正常对照组比较,PD组和20、50 mg/L臭氧治疗组大鼠黑质区GSH-Px活性均降低,MDA含量均增高;与PD组(GSH-Px:6.98±0.60,单位:U/mg·prot;MDA:2.31±0.54,单位:nmoL/mg· prot)比较,20 mg/L臭氧治疗组和50mg/L臭氧治疗组大鼠黑质区GSH-Px活性(9.37±0.41、10.04±0.59)升高,MDA含量(1.85±0.29、1.79±0.39)降低,差异均有统计学意义(P＜0.05).Western blotting检测结果显示4组大鼠黑质凋亡相关蛋白Bax和Bcl-2的表达比较差异无统计学意义(P＞0.05). 结论臭氧可以通过提高黑质部位GSH-Px活性、降低MDA含量而增强PD大鼠的抗氧化能力,但其对黑质凋亡相关蛋白Bax和Bcl-2的表达无明显影响.
목적 탐토취양대파금삼병(PD)대서모형흑질양화응격급조망상관단백표체적영향. 방법 SD대서32지안수궤수자표법분위PD조、20 mg/L취양치료조、50 mg/L취양치료조화정상대조조,매조8지,전3조대서우측문상체주사15μg6-간다파알(6-OHDA)제비PD모형,정상대조조주사등량생리염수;20、50 mg/L취양치료조분별재조모후4주경직장취입5.0 mL양기화취양적혼합기체(취양농도분별위20 mg/L화50 mg/L,제량위1 mg/kg),련속15d.상응처리후취대서중뇌흑질뇌조직,채용화학비색법측정흑질구지질과양화산물병이철(MDA)、초양화물기화매(SOD)화곡광감태초양화물매(GSH-Px)적농도;채용Western blotting검측흑질내조망상관단백B세포림파류기인-2(Bcl-2)화Bcl-2상관X단백(Bax)적표체. 결과 여정상대조조비교,PD조화20、50 mg/L취양치료조대서흑질구GSH-Px활성균강저,MDA함량균증고;여PD조(GSH-Px:6.98±0.60,단위:U/mg·prot;MDA:2.31±0.54,단위:nmoL/mg· prot)비교,20 mg/L취양치료조화50mg/L취양치료조대서흑질구GSH-Px활성(9.37±0.41、10.04±0.59)승고,MDA함량(1.85±0.29、1.79±0.39)강저,차이균유통계학의의(P＜0.05).Western blotting검측결과현시4조대서흑질조망상관단백Bax화Bcl-2적표체비교차이무통계학의의(P＞0.05). 결론 취양가이통과제고흑질부위GSH-Px활성、강저MDA함량이증강PD대서적항양화능력,단기대흑질조망상관단백Bax화Bcl-2적표체무명현영향.
Objective To investigate the effects of different concentrations of ozone on nigra oxidative stress and apoptosis-related protein expression in rats with Parkinson's disease (PD).Methods Thirty-two adult male SD rats aged 2-3 months and weighted 180-220 g were randomly divided into 4 groups (n=8):PD group,PD+ozone 20 group (given ozone 20 mg/L),PD+ozone 50 group (given ozone 50 mg/L) and control group; rats in the former three groups were given right striatum injection of 15 μg 6-OHDA to induce PD models,and rats in the control group were given the same volume of normal saline; four weeks after PD inducement,mixed gases of 5.0 mL of oxygen and ozone (20 mg/L and 50 mg/L,1 mg/kg) were given to rats in the PD+ozone 20 group and PD+ozone 50 group,respectively,for 15 d.Then,tissues were obtained from nigra; the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px),and the content of malondialdehyde (MDA) were measured by using chromometry; the protein expressions of B cell lymphoma/lewkmia-2 (Bcl-2) and Bcl-2 associated X protein (Bax) were detected by using Western blotting.Results As compared with those in the control group,the GSH-Px activity was significantly decreased and the MDA content was significantly increased in PD group,PD+ozone 20 group and PD+ozone 50 group (P＜0.05); the GSH-Px activity (6.98±0.60 vs 9.37±0.41、10.04±0.59) was significantly increased and the MDA content(2.31±0.54 vs 1.85±0.29、1.79±0.39) was significantly decreased in PD+ozone 20 group and PD+ozone 50 group as compared with those in the PD group (P＜0.05).Western blotting indicated no significant differences of protein expressions of Bax and Bcl-2 among the four groups (P＞0.05).Conclusion Ozone can improve antioxidation capability of rats with PD by increasing the activity of GSH-PX,while it does not affect the expression levels of Bax and Bcl-2.