目的 探讨富氢盐水对重度烫伤延迟复苏大鼠肝脏的影响. 方法 取24只SD大鼠,应用台式控温烫伤仪制成40％ TBSAⅢ度烫伤模型,按随机数字表法分为富氢盐水组、乳酸钠林格液组,每组12只.伤后6h开始,2组大鼠分别采用富氢盐水(自制,浓度为0.6 mmol/L)、乳酸钠林格液,均按4 mL· kg-1·％TBSA-1进行腹腔注射补液,持续到伤后48 h,伤后第2个24 h补液量为伤后第1个24 h的一半.伤后6(补液前)、12、24、48 h,每组分别取3只大鼠行心脏采血,处死后取肝组织.HE染色观察肝组织病理学变化,细胞计数板行肝组织中性粒细胞计数,全自动生化分析仪检测血清AST、ALT水平,ELISA法检测肝组织中TNF-α、IL-1β、IL-6以及8羟基脱氧鸟苷(8-OHdG)的含量,分光光度计检测肝组织中丙二醛的吸光度值并定量.对数据行重复测量方差分析和LSD-t检验. 结果 伤后48 h,与乳酸钠林格液组比较,富氢盐水组大鼠肝组织炎性细胞浸润减少,肝细胞充血减轻.伤后12、24、48 h,富氢盐水组大鼠肝组织中性粒细胞数分别为(25.3±1.8) ×105、(19.6±0.6) ×105、(14.1±3.2)×105个/mL,显著低于乳酸钠林格液组的(31.9±2.0)×105、(30.9±2.2) ×105、(23.8±3.0) ×105个/mL(t值分别为5.6、7.6、8.7,P值均小于0.05).伤后6、12h,2组大鼠血清AST、ALT水平以及肝组织TNF-α、IL-1 β、IL-6含量均相近(t值分别0.3 ～3.9与0.9 ～3.8,P值均大于0.05).伤后24、48 h,富氢盐水组大鼠血清AST与ALT水平分别为(308 ±24)、(210±15) U/L与(93±7)、(70 ±5) U/L,显著低于乳酸钠林格液组的(541 ±39)、(505±18)U/L与(156 ±9)、(166 ±21) U/L(t值分别为17.5、16.7与30.3、6.9,P值均小于0.05).伤后24、48 h,富氢盐水组大鼠肝组织TNF-α、1L-1 β、IL-6含量分别为(20.7±1.6)、(13.7±1.5)pg/mg,(7.7±1.5)、(6.3±1.2)pg/mg与(8.7±1.2)、(6.0±2.0)pg/mg,显著低于乳酸钠林格液组的(32.7±5.0)、(25.7±4.0)pg/mg,(16.3±2.5)、(12.0±2.7) pg/mg与(14.7±2.1)、(13.3±1.5) pg/mg(t值分别为5.2、5.7,4.7、4.7与10.4、4.4,P值均小于0.05).2组大鼠伤后6h肝组织丙二醛水平及伤后6、12h肝组织8-OHdG水平均相近(t值分别为0.1与0.7、4.3,P值均大于0.05).富氢盐水组大鼠伤后12、24、48 h肝组织丙二醛水平分别为(15.3±1.5)、(8.7±1.2)、(6.7±1.5) mmol/mg,显著低于乳酸钠林格液组的(27.3±4.7)、(20.3±1.5)、(14.0±1.0) mmol/mg(t值分别为5.2、5.7、5.1,P值均小于0.05);伤后24、48 h肝组织8-OHdG水平分别为(124±12)、(79±10) pg/mg,显著低于乳酸钠林格液组的(191±10)、(136±15) pg/mg(t值分别为8.0与8.1,P值均小于0.05). 结论 富氢盐水能够显著减轻重度烫伤延迟复苏大鼠肝脏中性粒细胞浸润,降低肝脏炎症因子水平,有效减轻肝脏氧化应激反应,进而保护大鼠肝脏.
目的 探討富氫鹽水對重度燙傷延遲複囌大鼠肝髒的影響. 方法 取24隻SD大鼠,應用檯式控溫燙傷儀製成40％ TBSAⅢ度燙傷模型,按隨機數字錶法分為富氫鹽水組、乳痠鈉林格液組,每組12隻.傷後6h開始,2組大鼠分彆採用富氫鹽水(自製,濃度為0.6 mmol/L)、乳痠鈉林格液,均按4 mL· kg-1·％TBSA-1進行腹腔註射補液,持續到傷後48 h,傷後第2箇24 h補液量為傷後第1箇24 h的一半.傷後6(補液前)、12、24、48 h,每組分彆取3隻大鼠行心髒採血,處死後取肝組織.HE染色觀察肝組織病理學變化,細胞計數闆行肝組織中性粒細胞計數,全自動生化分析儀檢測血清AST、ALT水平,ELISA法檢測肝組織中TNF-α、IL-1β、IL-6以及8羥基脫氧鳥苷(8-OHdG)的含量,分光光度計檢測肝組織中丙二醛的吸光度值併定量.對數據行重複測量方差分析和LSD-t檢驗. 結果 傷後48 h,與乳痠鈉林格液組比較,富氫鹽水組大鼠肝組織炎性細胞浸潤減少,肝細胞充血減輕.傷後12、24、48 h,富氫鹽水組大鼠肝組織中性粒細胞數分彆為(25.3±1.8) ×105、(19.6±0.6) ×105、(14.1±3.2)×105箇/mL,顯著低于乳痠鈉林格液組的(31.9±2.0)×105、(30.9±2.2) ×105、(23.8±3.0) ×105箇/mL(t值分彆為5.6、7.6、8.7,P值均小于0.05).傷後6、12h,2組大鼠血清AST、ALT水平以及肝組織TNF-α、IL-1 β、IL-6含量均相近(t值分彆0.3 ～3.9與0.9 ～3.8,P值均大于0.05).傷後24、48 h,富氫鹽水組大鼠血清AST與ALT水平分彆為(308 ±24)、(210±15) U/L與(93±7)、(70 ±5) U/L,顯著低于乳痠鈉林格液組的(541 ±39)、(505±18)U/L與(156 ±9)、(166 ±21) U/L(t值分彆為17.5、16.7與30.3、6.9,P值均小于0.05).傷後24、48 h,富氫鹽水組大鼠肝組織TNF-α、1L-1 β、IL-6含量分彆為(20.7±1.6)、(13.7±1.5)pg/mg,(7.7±1.5)、(6.3±1.2)pg/mg與(8.7±1.2)、(6.0±2.0)pg/mg,顯著低于乳痠鈉林格液組的(32.7±5.0)、(25.7±4.0)pg/mg,(16.3±2.5)、(12.0±2.7) pg/mg與(14.7±2.1)、(13.3±1.5) pg/mg(t值分彆為5.2、5.7,4.7、4.7與10.4、4.4,P值均小于0.05).2組大鼠傷後6h肝組織丙二醛水平及傷後6、12h肝組織8-OHdG水平均相近(t值分彆為0.1與0.7、4.3,P值均大于0.05).富氫鹽水組大鼠傷後12、24、48 h肝組織丙二醛水平分彆為(15.3±1.5)、(8.7±1.2)、(6.7±1.5) mmol/mg,顯著低于乳痠鈉林格液組的(27.3±4.7)、(20.3±1.5)、(14.0±1.0) mmol/mg(t值分彆為5.2、5.7、5.1,P值均小于0.05);傷後24、48 h肝組織8-OHdG水平分彆為(124±12)、(79±10) pg/mg,顯著低于乳痠鈉林格液組的(191±10)、(136±15) pg/mg(t值分彆為8.0與8.1,P值均小于0.05). 結論 富氫鹽水能夠顯著減輕重度燙傷延遲複囌大鼠肝髒中性粒細胞浸潤,降低肝髒炎癥因子水平,有效減輕肝髒氧化應激反應,進而保護大鼠肝髒.
목적 탐토부경염수대중도탕상연지복소대서간장적영향. 방법 취24지SD대서,응용태식공온탕상의제성40％ TBSAⅢ도탕상모형,안수궤수자표법분위부경염수조、유산납림격액조,매조12지.상후6h개시,2조대서분별채용부경염수(자제,농도위0.6 mmol/L)、유산납림격액,균안4 mL· kg-1·％TBSA-1진행복강주사보액,지속도상후48 h,상후제2개24 h보액량위상후제1개24 h적일반.상후6(보액전)、12、24、48 h,매조분별취3지대서행심장채혈,처사후취간조직.HE염색관찰간조직병이학변화,세포계수판행간조직중성립세포계수,전자동생화분석의검측혈청AST、ALT수평,ELISA법검측간조직중TNF-α、IL-1β、IL-6이급8간기탈양조감(8-OHdG)적함량,분광광도계검측간조직중병이철적흡광도치병정량.대수거행중복측량방차분석화LSD-t검험. 결과 상후48 h,여유산납림격액조비교,부경염수조대서간조직염성세포침윤감소,간세포충혈감경.상후12、24、48 h,부경염수조대서간조직중성립세포수분별위(25.3±1.8) ×105、(19.6±0.6) ×105、(14.1±3.2)×105개/mL,현저저우유산납림격액조적(31.9±2.0)×105、(30.9±2.2) ×105、(23.8±3.0) ×105개/mL(t치분별위5.6、7.6、8.7,P치균소우0.05).상후6、12h,2조대서혈청AST、ALT수평이급간조직TNF-α、IL-1 β、IL-6함량균상근(t치분별0.3 ～3.9여0.9 ～3.8,P치균대우0.05).상후24、48 h,부경염수조대서혈청AST여ALT수평분별위(308 ±24)、(210±15) U/L여(93±7)、(70 ±5) U/L,현저저우유산납림격액조적(541 ±39)、(505±18)U/L여(156 ±9)、(166 ±21) U/L(t치분별위17.5、16.7여30.3、6.9,P치균소우0.05).상후24、48 h,부경염수조대서간조직TNF-α、1L-1 β、IL-6함량분별위(20.7±1.6)、(13.7±1.5)pg/mg,(7.7±1.5)、(6.3±1.2)pg/mg여(8.7±1.2)、(6.0±2.0)pg/mg,현저저우유산납림격액조적(32.7±5.0)、(25.7±4.0)pg/mg,(16.3±2.5)、(12.0±2.7) pg/mg여(14.7±2.1)、(13.3±1.5) pg/mg(t치분별위5.2、5.7,4.7、4.7여10.4、4.4,P치균소우0.05).2조대서상후6h간조직병이철수평급상후6、12h간조직8-OHdG수평균상근(t치분별위0.1여0.7、4.3,P치균대우0.05).부경염수조대서상후12、24、48 h간조직병이철수평분별위(15.3±1.5)、(8.7±1.2)、(6.7±1.5) mmol/mg,현저저우유산납림격액조적(27.3±4.7)、(20.3±1.5)、(14.0±1.0) mmol/mg(t치분별위5.2、5.7、5.1,P치균소우0.05);상후24、48 h간조직8-OHdG수평분별위(124±12)、(79±10) pg/mg,현저저우유산납림격액조적(191±10)、(136±15) pg/mg(t치분별위8.0여8.1,P치균소우0.05). 결론 부경염수능구현저감경중도탕상연지복소대서간장중성립세포침윤,강저간장염증인자수평,유효감경간장양화응격반응,진이보호대서간장.
Objective To explore the effects of hydrogen-rich saline (HS) on liver of severely scalded rats with delayed resuscitation.Methods Twenty-four SD rats were inflicted with 40％ TBSA fullthickness scald using a temperature-controlled scalding apparatus.The injured rats were divided into lactated Ringer's solution (RS) and HS groups according to the random number table,with 12 rats in each group.Rats in groups RS and HS were respectively resuscitated with an intraperitoneal injection of 4 mL · kg-1 · ％ TBSA-1 of RS or HS (self-prepared,with concentration of hydrogen 0.6 mmol/L) 6 hours after injury up to 48 hours after scald.The infusion volume of the second 24 hours after injury was a half of that of the first 24 hours.At post scald hour (PSH) 6 (before resuscitation),12,24,and 48,blood was collected from the heart of 3 rats in each group,and then the rats were sacrificed for harvesting liver tissue.The pathological change in liver tissue was observed with HE staining.The number of hepatic neutrophils was counted with a hematocytometer.Serum levels of AST and ALT were determined with full-automatic biochemical analyzer.Contents of TNF-α,IL-1β,IL-6,and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in liver tissue were determined with ELISA.Absorbance value of malondialdehyde (MDA) in liver tissue was detected and quantified with spectrophotometer.Data were processed with analysis of variance of repeated measurement and LSD-t test.Results At PSH 48,moderate infiltration of inflammatory cells and hepatic hyperemia were observed in rats of group HS as compared with group RS.At PSH 12,24,and 48,the number of neutrophils in group HS was respectively (25.3 ± 1.8) × 105,(19.6 ± 0.6) × 105,and (14.1 ± 3.2) × 105 cells per mililitre,and they were significantly lower than those in group RS [(31.9 ± 2.0) × 105,(30.9 ± 2.2) × 105,and (23.8 ±3.0) × 105cells per mililitre,with t values respectively 5.6,7.6,and 8.7,P values below 0.05].At PSH 6 and 12,the serum levels of AST and ALT and the levels of TNF-α,IL-1β,and IL-6 in liver tissue were close between the two groups (with t values respectively 0.3-3.9 and 0.9-3.8,P values above 0.05).At PSH 24 and 48,the serum levels of AST and ALT in group HS were respectively (308 ±24) and (210 ± 15) U/L and (93 ±7) and (70 ±5) U/L,which were significantly lower than those in group RS [(541 ±39) and (505 ± 18) U/L,with t values respectively 17.5 and 16.7,P values below 0.05; (156 ± 9) and (166 ± 21) U/L,with t values respectively 30.3 and 6.9,P values below 0.05].At PSH 24 and 48,the levels of TNF-α,IL-1 β,and IL-6 in liver tissue in group HS were respectively (20.7 ± 1.6) and (13.7 ±1.5) pg/mg,(7.7±1.5) and (6.3±1.2) pg/mg,and (8.7±1.2) and (6.0±2.0) pg/mg,which were significantly lower than those in group RS [(32.7 ± 5.0) and (25.7 ± 4.0) pg/mg,with t values respectively 5.2 and 5.7,P values below 0.05 ; (16.3 ± 2.5) and (12.0 ± 2.7) pg/mg,with t values both as 4.7,P values below 0.05 ; (14.7 ± 2.1) and (13.3 ± 1.5) pg/mg,with t values respectively 10.4 and 4.4,P values below 0.05].The level of MDA at PSH 6 and levels of 8-OHdG at PSH 6 and 12 in liver tissue were close between the two groups (with t values respectively 0.1,0.7,and 4.3,P values above 0.05).In group HS,the levels of MDA in liver tissue at PSH 12,24,and 48 were respectively (15.3 ± 1.5),(8.7 ± 1.2),and (6.7 ± 1.5) mmol/mg,and the levels of hepatic 8-OHdG at PSH 24 and 48 were respectively (124 ± 12) and (79 ± 10) pg/mg,which were significantly lower than those in group RS [(27.3±4.7),(20.3±1.5),and (14.0±1.0) mmol/mg,with t values respectively 5.2,5.7,and 5.1,P values below 0.05 ; (191 ± 10) and (136 ± 15) pg/mg,with t values respectively 8.0 and 8.1,P values below 0.05].Conclusions Resuscitation with HS could protect liver of severely scalded rats with delayed resuscitation possibly by reducing infiltration of neutrophils,thus lowering the content of inflammatory cytokines,and effectively alleviating oxidative stress.
