CAJ | 학술논문

目的了解健康人及慢性鼻-鼻窦炎(chronic rhinosinusitis,CRS)患者鼻腔黏膜上皮细胞分泌的补体调节蛋白CD55及CD59的表达情况及其意义.方法采用细胞培养及流式细胞仪等方法检测健康对照组(30例)及CRS患者(30例)鼻腔黏膜上皮细胞分泌的补体调节蛋白CD55及CD59的相对表达量.以SPSS 18.0软件进行统计学分析.结果 CD55在健康对照组鼻腔黏膜组织中的表达量为0.001 ±0.001,低于其在CRS组鼻腔黏膜组织中的表达量(0.067±0.028),差异有统计学意义(t=-10.535,P＜0.01).CD59在健康对照组鼻腔黏膜组织的表达量为0.879±0.005,高于其在CRS组中的表达量(0.238±0.034),差异有统计学意义(t=83.416,P＜0.01).结论 CRS患者鼻黏膜组织中CD55呈高表达而CD59呈低表达,这一机制可能参与了CRS的发病过程.
목적 료해건강인급만성비-비두염(chronic rhinosinusitis,CRS)환자비강점막상피세포분비적보체조절단백CD55급CD59적표체정황급기의의.방법 채용세포배양급류식세포의등방법검측건강대조조(30례)급CRS환자(30례)비강점막상피세포분비적보체조절단백CD55급CD59적상대표체량.이SPSS 18.0연건진행통계학분석.결과 CD55재건강대조조비강점막조직중적표체량위0.001 ±0.001,저우기재CRS조비강점막조직중적표체량(0.067±0.028),차이유통계학의의(t=-10.535,P＜0.01).CD59재건강대조조비강점막조직적표체량위0.879±0.005,고우기재CRS조중적표체량(0.238±0.034),차이유통계학의의(t=83.416,P＜0.01).결론 CRS환자비점막조직중CD55정고표체이CD59정저표체,저일궤제가능삼여료CRS적발병과정.
Objective To understand the expression of complement regulatory proteins CD55 and CD59,which secreted by epithelial cells of normal and chronic sinusitis patients.Methods Cell culture and flow cytometry were used to detect the expression of complement regulatory proteins CD55 and CD59.SPSS 18.0 software was used to analyze the data.Results CD55 was expressed both in normal nasal mucosa and mucosa of chronic sinusitis.The expression in normal group was 0.001 ±0.001,significantly lower than that in CRS group which was 0.067 ± 0.028 (t =-10.535,P ＜ 0.01).CD59 was also expressed in the two groups.In normal group,the expression of CD59 was 0.879 ± 0.005,significantly higher than that in CRS group which was 0.238 ± 0.034 (t =83.416,P ＜0.01).Conclusions The nasal mucosa in CRS patients showed low expression of CD55 and high expression of CD59.This mechanism may be involved in the occurrence of CRS.