仲恺农业工程学院学报
仲愷農業工程學院學報
중개농업공정학원학보
JOURNAL OF ZHONGKAI UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
2014年
4期
25-29
,共5页
蓖麻毒蛋白%筛选%解毒剂%中草药
蓖痳毒蛋白%篩選%解毒劑%中草藥
비마독단백%사선%해독제%중초약
ricin%screening%antidote%Chinese medicine herbs
为了从31种中草药中筛选出对蓖麻粗毒蛋白具有较好解毒效果的中草药提取物，采用5种不同提取方法提取中草药有效成分，以小白鼠为试验对象，就31种中草药乙醇提取物对蓖麻毒蛋白中毒小鼠的解毒效果进行了粗筛，并就5种不同提取方法提取中草药提取液对蓖麻毒蛋白中毒小鼠的解毒效果进行了比较。结果表明，31种中草药乙醇提取物中，筛选出解毒效果较好的大黄、连翘、板蓝根、防风、白芍、附子、龙血竭、升麻（广东）和升麻（西安）等9种中草药；又从这9种中草药乙醇提取物中进一步筛选出解毒效果好且效果稳定的板蓝根、白芍、龙血竭和升麻（西安）等4种中草药；在5种提取方法中，以超声波丙酮提取法提取的中草药的解毒效果最好，超声波乙醇提取法次之，超声波水提取法效果最差；而在板蓝根、白芍、龙血竭和升麻（西安）等4种中草药提取液中，则以白芍提取液的解毒效果最佳，升麻（西安）的解毒效果最差。由此可以证明，白芍，龙血竭，板蓝根和升麻（西安）为极具潜力的蓖麻粗毒蛋白中草药解毒剂。
為瞭從31種中草藥中篩選齣對蓖痳粗毒蛋白具有較好解毒效果的中草藥提取物，採用5種不同提取方法提取中草藥有效成分，以小白鼠為試驗對象，就31種中草藥乙醇提取物對蓖痳毒蛋白中毒小鼠的解毒效果進行瞭粗篩，併就5種不同提取方法提取中草藥提取液對蓖痳毒蛋白中毒小鼠的解毒效果進行瞭比較。結果錶明，31種中草藥乙醇提取物中，篩選齣解毒效果較好的大黃、連翹、闆藍根、防風、白芍、附子、龍血竭、升痳（廣東）和升痳（西安）等9種中草藥；又從這9種中草藥乙醇提取物中進一步篩選齣解毒效果好且效果穩定的闆藍根、白芍、龍血竭和升痳（西安）等4種中草藥；在5種提取方法中，以超聲波丙酮提取法提取的中草藥的解毒效果最好，超聲波乙醇提取法次之，超聲波水提取法效果最差；而在闆藍根、白芍、龍血竭和升痳（西安）等4種中草藥提取液中，則以白芍提取液的解毒效果最佳，升痳（西安）的解毒效果最差。由此可以證明，白芍，龍血竭，闆藍根和升痳（西安）為極具潛力的蓖痳粗毒蛋白中草藥解毒劑。
위료종31충중초약중사선출대비마조독단백구유교호해독효과적중초약제취물，채용5충불동제취방법제취중초약유효성분，이소백서위시험대상，취31충중초약을순제취물대비마독단백중독소서적해독효과진행료조사，병취5충불동제취방법제취중초약제취액대비마독단백중독소서적해독효과진행료비교。결과표명，31충중초약을순제취물중，사선출해독효과교호적대황、련교、판람근、방풍、백작、부자、룡혈갈、승마（엄동）화승마（서안）등9충중초약；우종저9충중초약을순제취물중진일보사선출해독효과호차효과은정적판람근、백작、룡혈갈화승마（서안）등4충중초약；재5충제취방법중，이초성파병동제취법제취적중초약적해독효과최호，초성파을순제취법차지，초성파수제취법효과최차；이재판람근、백작、룡혈갈화승마（서안）등4충중초약제취액중，칙이백작제취액적해독효과최가，승마（서안）적해독효과최차。유차가이증명，백작，룡혈갈，판람근화승마（서안）위겁구잠력적비마조독단백중초약해독제。
In order to screen out Chinese herb extracts from 31 kinds of Chinese medicinal herbs herbal which have good antidotal effect on raw ricin , five different extraction methods were investigated .Mice were used as experimental subjects , 31 kinds of herbal ethanol extracts were used to sieve coarsely by the detoxifying effect of ricin poisoning on mice .The detoxifying effects of the Chinese herb extracts extracted by five different methods were compared on ricin poisoned mice .The results showed that nine kinds of Chinese medicinal herbs were found to have good detoxification from 31 kinds of herbal ethanol extracts , for example Rhubarb , Forsythia , Radix , Divaricate saposhnikovia root , White paeony root , Radix aconiti lateralis preparata , Resina draconis , Cimicifuga ( Guangdong ) and Cimicifuga ( Xi'an ); and that four kinds of Chinese medicinal herbs were screened out to have good and stable detoxifying effect from the nine kinds of herbal ethanol extracts , including Radix , White paeony root , Resina draconis and Cimicifu-ga ( Xi'an) .In the five kinds of extraction methods , ultrasonic acetone extraction method followed by the ultrasonic ethanol extraction method and the ultrasonic water extraction method .In Radix, White paeony root, Resina draconis and Cimicifuga ( Xi'an) four kinds of Chinese herb extracts , White paeony root was found to be the best antidote , and Cimicifuga ( Xi'an) was found to be the worst .The results demonstra-ted that Radix, White paeony root, Resina draconis and Cimicifuga (Xi'an) could be used for potential antidotes .
