仲恺农业工程学院学报
仲愷農業工程學院學報
중개농업공정학원학보
JOURNAL OF ZHONGKAI UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
2014年
4期
20-24
,共5页
豆豉曲%米曲霉(Aspergillus oryzae)%诱变%酶活
豆豉麯%米麯黴(Aspergillus oryzae)%誘變%酶活
두시곡%미곡매(Aspergillus oryzae)%유변%매활
koji-making Douchi%Aspergillus oryzae%mutagenesis%protease activity
从曲霉型阳江豆豉曲中筛选出5株菌落形态不同的曲霉（ Aspergillus spp．），通过纯种发酵制备豆豉曲，测定了豆豉曲中碱性蛋白酶和中性蛋白酶的活性，从中筛选出1株蛋白酶活性较高的菌种，并对该曲霉进行ITS rDNA序列分析，发现该曲霉与Aspergillus oryzae strain P6B2（JX429926）的同源性最高，故鉴定为米曲霉（As-pergillus oryzae）．以该米曲霉为出发菌株，通过紫外线诱变和硫酸二乙酯化学复合诱变处理，选育得到优良高产蛋白酶活性的突变株，其中性蛋白酶酶活达258 U／g，比出发菌株（210 U／g）高出22.9％，且具有良好的遗传稳定性．
從麯黴型暘江豆豉麯中篩選齣5株菌落形態不同的麯黴（ Aspergillus spp．），通過純種髮酵製備豆豉麯，測定瞭豆豉麯中堿性蛋白酶和中性蛋白酶的活性，從中篩選齣1株蛋白酶活性較高的菌種，併對該麯黴進行ITS rDNA序列分析，髮現該麯黴與Aspergillus oryzae strain P6B2（JX429926）的同源性最高，故鑒定為米麯黴（As-pergillus oryzae）．以該米麯黴為齣髮菌株，通過紫外線誘變和硫痠二乙酯化學複閤誘變處理，選育得到優良高產蛋白酶活性的突變株，其中性蛋白酶酶活達258 U／g，比齣髮菌株（210 U／g）高齣22.9％，且具有良好的遺傳穩定性．
종곡매형양강두시곡중사선출5주균락형태불동적곡매（ Aspergillus spp．），통과순충발효제비두시곡，측정료두시곡중감성단백매화중성단백매적활성，종중사선출1주단백매활성교고적균충，병대해곡매진행ITS rDNA서렬분석，발현해곡매여Aspergillus oryzae strain P6B2（JX429926）적동원성최고，고감정위미곡매（As-pergillus oryzae）．이해미곡매위출발균주，통과자외선유변화류산이을지화학복합유변처리，선육득도우량고산단백매활성적돌변주，기중성단백매매활체258 U／g，비출발균주（210 U／g）고출22.9％，차구유량호적유전은정성．
A higher protease-producing strain was selected within 5 different strains of Aspergillus spp.i-solated from Aspergillus of koji-making Douchi type of Yangjiang by testing the alkaline and neutral prote-ase activity with the purebred fermentation of koji-making Douchi .The strain was most highly homologous with Aspergillus oryzae strain P6B2 ( JX429926) and identified as Aspergillus oryzae.With the Aspergillus oryzae as a start strain , an excellent mutant strain was mutated multiply by UV and diethyl sulfate through separation , purification and cultivation .Its neutral protease activity was 258 U/g, which was increased by 22.9%compared to that of the original strain (210 U/g) , with stable hereditary character .
