CAJ | 학술논문

目的：建立并鉴定CLCN3／MMTV－PyMT双转基因小鼠杂交群体，构建氯通道ClC－3过表达的自发乳腺肿瘤转基因小鼠模型。方法将CLCN3转基因小鼠和MMTV－PyMT转基因小鼠分别进行繁殖，选取阳性子代鼠进行配对，培育杂交后代，然后通过PCR鉴定基因型、通过组织免疫荧光方法、Western blot检测转基因小鼠蛋白表达情况。结果成功繁育CLCN3转基因小鼠和 MMTV－PyMT转基因小鼠，经PCR鉴定成功构建 CLCN3／MMTV－PyMT双转基因鼠杂交群体，并成功保种和扩群，经组织免疫荧光和Western－blot分析双转基因小鼠的ClC－3蛋白的表达高于MMTV－PyMT转基因小鼠。结论成功构建ClC－3过表达的自发乳腺肿瘤转基因小鼠模型，为进一步研究ClC－3在肿瘤生长和转移中的功能提供了良好的动物模型。
목적：건립병감정CLCN3／MMTV－PyMT쌍전기인소서잡교군체，구건록통도ClC－3과표체적자발유선종류전기인소서모형。방법장CLCN3전기인소서화MMTV－PyMT전기인소서분별진행번식，선취양성자대서진행배대，배육잡교후대，연후통과PCR감정기인형、통과조직면역형광방법、Western blot검측전기인소서단백표체정황。결과성공번육CLCN3전기인소서화 MMTV－PyMT전기인소서，경PCR감정성공구건 CLCN3／MMTV－PyMT쌍전기인서잡교군체，병성공보충화확군，경조직면역형광화Western－blot분석쌍전기인소서적ClC－3단백적표체고우MMTV－PyMT전기인소서。결론성공구건ClC－3과표체적자발유선종류전기인소서모형，위진일보연구ClC－3재종류생장화전이중적공능제공료량호적동물모형。
Objective To establish a CLCN3/MMTV-PyMT double transgenic mouse model of spontaneous breast tumor with simultaneously overexpressing ClC-3.Method CLCN3 transgenic mice were crossed with MMTV-PyMT spon-taneous mammary tumor model mice.The genotype was determined by PCR.The expression of ClC-3 in tissues was detec-ted by immunofluorescence and Western blot.Results CLCN3 and MMTV-PyMT transgenic mice were bred and CLCN3/MMTV-PyMT hybrid mouse model was successfully established.The ClC-3 expression in CLCN3/MMTV-PyMT hybrid mice was higher than that in the MMTV-PyMT mice, assessed by immunofluorescence and Western blot analysis.Conclu-sions Transgenic mouse models of spontaneous breast cancer with simultaneously overexpressing ClC-3 are successfully es-tablished.The double transgenic mice provide a good animal model for further research of ClC-3 in tumor growth and metas-tasis.