中国实验动物学报
中國實驗動物學報
중국실험동물학보
ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA
2014年
6期
85-88
,共4页
梁德%唐晶晶%江晓兵%姚珍松%张顺聪%杨志东%魏秋实%崔健超%任辉%沈耿杨%林顺鑫
樑德%唐晶晶%江曉兵%姚珍鬆%張順聰%楊誌東%魏鞦實%崔健超%任輝%瀋耿楊%林順鑫
량덕%당정정%강효병%요진송%장순총%양지동%위추실%최건초%임휘%침경양%림순흠
不同给予途径%泼尼松%大鼠%骨密度%骨生物力学%骨代谢
不同給予途徑%潑尼鬆%大鼠%骨密度%骨生物力學%骨代謝
불동급여도경%발니송%대서%골밀도%골생물역학%골대사
Prednisone%Oral administration%Intramuscular injection%Rat%Bone mineral density%Skeletal bio-mechanical properties%Bone metabolism
目的：观察泼尼松灌胃与肌内注射两种不同给药方法对大鼠骨密度、骨生物力学及骨代谢的影响。方法将45只SPF级雄性SD大鼠随机分为3组（正常组15只、灌胃组15只、肌内注射组15只），其中正常组大鼠作为阴性对照，予0．9％生理盐水灌胃2 mL／d；灌胃组大鼠给予泼尼松0．5 mg／（ kg?d）灌胃；肌内注射组大鼠给予泼尼松0．5 mg／（ kg?d）；12周后测定离体的大鼠椎体骨密度及血清β－CTX、PINP水平变化，采用三点弯曲试验测量股骨皮质骨最大载荷、弹性载荷、断裂载荷等生物力学指标。结果与正常组相比，灌胃组及肌内注射组大鼠椎骨骨密度值均显著性降低（P＜0．05）；与灌胃组相比，肌内注射组大鼠椎骨骨密度显著下降（P＜0．05）；与正常组相比，灌胃组及肌内注射组大鼠股骨的弹性载荷、最大载荷、断裂载荷均显著降低（ P＜0．05），肌内注射组与灌胃组大鼠的弹性载荷、最大载荷、断裂载荷相比差异无显著性（ P＞0．05）。与正常组相比，灌胃组及肌内注射组大鼠中血清β－CTX水平均显著升高（P＜0．05）而PINP水平均显著降低（P＜0．05），与灌胃组相比，肌内注射组大鼠血清β－CTX水平显著升高（P＜0．05）而PINP水平显著降低（P＜0．05）。骨组织切片HE染色显示：肌内注射组大鼠的骨小梁明显纤细疏松，造血组织明显减少，脂肪组织明显增多。结论泼尼松对大鼠的骨密度、骨生物力学及骨代谢指标都有影响，而肌内注射泼尼松比口服对骨密度、骨强度、骨代谢的影响更大，更易造成骨质疏松症。因此，建议临床使用泼尼松时选择口服作为给药方式更安全。
目的：觀察潑尼鬆灌胃與肌內註射兩種不同給藥方法對大鼠骨密度、骨生物力學及骨代謝的影響。方法將45隻SPF級雄性SD大鼠隨機分為3組（正常組15隻、灌胃組15隻、肌內註射組15隻），其中正常組大鼠作為陰性對照，予0．9％生理鹽水灌胃2 mL／d；灌胃組大鼠給予潑尼鬆0．5 mg／（ kg?d）灌胃；肌內註射組大鼠給予潑尼鬆0．5 mg／（ kg?d）；12週後測定離體的大鼠椎體骨密度及血清β－CTX、PINP水平變化，採用三點彎麯試驗測量股骨皮質骨最大載荷、彈性載荷、斷裂載荷等生物力學指標。結果與正常組相比，灌胃組及肌內註射組大鼠椎骨骨密度值均顯著性降低（P＜0．05）；與灌胃組相比，肌內註射組大鼠椎骨骨密度顯著下降（P＜0．05）；與正常組相比，灌胃組及肌內註射組大鼠股骨的彈性載荷、最大載荷、斷裂載荷均顯著降低（ P＜0．05），肌內註射組與灌胃組大鼠的彈性載荷、最大載荷、斷裂載荷相比差異無顯著性（ P＞0．05）。與正常組相比，灌胃組及肌內註射組大鼠中血清β－CTX水平均顯著升高（P＜0．05）而PINP水平均顯著降低（P＜0．05），與灌胃組相比，肌內註射組大鼠血清β－CTX水平顯著升高（P＜0．05）而PINP水平顯著降低（P＜0．05）。骨組織切片HE染色顯示：肌內註射組大鼠的骨小樑明顯纖細疏鬆，造血組織明顯減少，脂肪組織明顯增多。結論潑尼鬆對大鼠的骨密度、骨生物力學及骨代謝指標都有影響，而肌內註射潑尼鬆比口服對骨密度、骨彊度、骨代謝的影響更大，更易造成骨質疏鬆癥。因此，建議臨床使用潑尼鬆時選擇口服作為給藥方式更安全。
목적：관찰발니송관위여기내주사량충불동급약방법대대서골밀도、골생물역학급골대사적영향。방법장45지SPF급웅성SD대서수궤분위3조（정상조15지、관위조15지、기내주사조15지），기중정상조대서작위음성대조，여0．9％생리염수관위2 mL／d；관위조대서급여발니송0．5 mg／（ kg?d）관위；기내주사조대서급여발니송0．5 mg／（ kg?d）；12주후측정리체적대서추체골밀도급혈청β－CTX、PINP수평변화，채용삼점만곡시험측량고골피질골최대재하、탄성재하、단렬재하등생물역학지표。결과여정상조상비，관위조급기내주사조대서추골골밀도치균현저성강저（P＜0．05）；여관위조상비，기내주사조대서추골골밀도현저하강（P＜0．05）；여정상조상비，관위조급기내주사조대서고골적탄성재하、최대재하、단렬재하균현저강저（ P＜0．05），기내주사조여관위조대서적탄성재하、최대재하、단렬재하상비차이무현저성（ P＞0．05）。여정상조상비，관위조급기내주사조대서중혈청β－CTX수평균현저승고（P＜0．05）이PINP수평균현저강저（P＜0．05），여관위조상비，기내주사조대서혈청β－CTX수평현저승고（P＜0．05）이PINP수평현저강저（P＜0．05）。골조직절편HE염색현시：기내주사조대서적골소량명현섬세소송，조혈조직명현감소，지방조직명현증다。결론발니송대대서적골밀도、골생물역학급골대사지표도유영향，이기내주사발니송비구복대골밀도、골강도、골대사적영향경대，경역조성골질소송증。인차，건의림상사용발니송시선택구복작위급약방식경안전。
Objective To compare the effects of gastric gavage and intramuscular injection of prednisone on the bone mineral density, skeletal biomechanical properties and bone metabolism in rats.Methods A total of 45 SPF rats were randomly divided into three groups:normal group, intragastric administration group, and intramuscular injection group.The normal group, as a control group, was administrated with normal saline 2 mL per day, both the intragastric administration group and i.m.injec-tion group received prednisone 0.5 mg/(kg.d) for 12 weeks.All rats were examined for bone mineral density (BMD) and the level of serum β-CTX and PINP.The femoral cortical biomechanical properties ( elastic load, maximal load, rupturing load) were measured by three point bending test.Results After 12 weeks, compared with the normal group, BMD and elastic load, maximal load, and rupturing load of the femur were significantly decreased.Compared with the intragastric gavage group, BMD was significantly decreased, while the elastic load, maximal load, and rupturing load of the femur were not significantly changed in the i.m.injection group (P<0.05 for all).Compared with the normal group, the level of serum β-CTX was significantly raised (P<0.05) and the level of serum PINP was significantly decreased (P<0.05).Compared with the intragastric gavage group, the level of serumβ-CTX was also significantly raised (P<0.05), the level of serum PINP was significantly decreased (P<0.05), the bone trabecula and hemopoietic tissue were obviously decreased, while the adipose tissue increased obviously. Conclusions Both intragastric gavage and intramuscular injection of prednisone affect the level of BMD, skeletal biomechanical properties and bone metabolism.However, i.m.injection of prednisone decreases the BMD and bone strength more significantly, leading to a higher bone turnover with increased bone resorption, and leads to osteoporosis earlier.Our results may suggest that oral administration of prednisone is more safe in clinical treatment.
