CAJ | 학술논문

建立了强化食品(饮料、奶粉、含乳饮料、大米、果泥及果冻)中维生素 C 含量的高效液相色谱检测方法。优化了样品处理方法，在水浴控温和避光条件下处理样品，避免维生素 C 被氧化。选用 TechMate C18–ST (250 mm×4.6 mm，5μm)反相色谱柱，以0.05 mol／L 磷酸二氢钾缓冲溶液(pH 3)为流动相，流量为1.0 mL／min，检测器为光电二极管阵列检测器，检测波长为266 nm。线性范围为0.2～100μg／mL，相关系数为0.9996，果泥中维生素 C 的定量限为20 mg／kg，其它为100 mg／kg，加标回收率为82.2%～107%，测定结果的相对标准偏差为1.23%～6.86%(n=8)。该方法简单快速，其灵敏度、准确度和精密度均能满足强化食品中维生素 C 的检测要求。
건립료강화식품(음료、내분、함유음료、대미、과니급과동)중유생소 C 함량적고효액상색보검측방법。우화료양품처리방법，재수욕공온화피광조건하처리양품，피면유생소 C 피양화。선용 TechMate C18–ST (250 mm×4.6 mm，5μm)반상색보주，이0.05 mol／L 린산이경갑완충용액(pH 3)위류동상，류량위1.0 mL／min，검측기위광전이겁관진렬검측기，검측파장위266 nm。선성범위위0.2～100μg／mL，상관계수위0.9996，과니중유생소 C 적정량한위20 mg／kg，기타위100 mg／kg，가표회수솔위82.2%～107%，측정결과적상대표준편차위1.23%～6.86%(n=8)。해방법간단쾌속，기령민도、준학도화정밀도균능만족강화식품중유생소 C 적검측요구。
A method for determination of vitamin C (Vc) in fortified foods (juice,milk power,milk drinks,rice, puree and jelly) by high performance liquid chromatography (HPLC) was established. The sample processing method was optimized by controlling the water bath tempertature and keeping in dark conditions to avoid oxidation of vitamin C. The samples were analyzed using 0.05 mol/L potassium dihydrogen phosphate buffer solution (pH 3) as mobile phase with the flow rate of 1.0 mL/min, and detected by means of photodiode array detector with the detection wavelength 266 nm. A good linear relationship was shown at the range of 0.2–100 μg/mL with the linear correlation coefficient of 0.999 6, and the detection limits were 100 mg/kg (20 mg/kg for puree). The recoveries were 82.2%–107%, and the relative standard deviation of determination results was 1.23%–6.86%(n=8). This method had the advantage of easy sample handling, high sensitivity and precision, which can meet the requirements of the determination of vitamin C in fortified foods.